Figure 3
From: Peroxiredoxins: hidden players in the antioxidant defence of human spermatozoa
Lipid peroxidation is not increased in human spermatozoa treated with 5 mM diethylmaleate (DEM; binds to GSH making it non-accessible for the GPX-GRD system), 50 μM carmustine (inactivates glutathione reductase and diaphorase activity) or 50 μM NaN 3 (inhibitor of catalase). Lipid peroxidation was measured by spectrofluorometry according to Aitken et al. (1993) [103]. Spermatozoa from 4 different healthy donors were used in this experiment. The presence of none of the inhibitors used increased the level of lipid peroxidation in human spermatozoa (results were analyzed by ANOVA; p < 0.05).