In Vitro fertilization failure of normozoospermic men: search for a lack of testicular isozyme of angiotensin-converting enzyme

In standard in vitro fertilization (IVF), 5 to 10% of patients have a complete failure of fertilization [1]. It may result from impaired spermatozoa, oocyte deficiencies or defects in the in vitro sperm-oocyte interaction. In assisted reproductive technology programs, 70% of mature oocytes will successfully fertilize. Thus, each oocyte has 30% of chance of fertilization failure and the likelihood of failed fertilization would depend on the number of available oocytes (n) and is (0.3)ⁿ. Thus, the chance of failed fertilization in a couple with three mature oocytes should be approximately 2.7% [1] and in these cases, sperm defects appear to be the major cause although semen parameters are normal. Strongly supporting this hypothesis is the observation that failed fertilized oocytes reincubated with donor spermatozoa usually fertilize [1]. This sperm defect can lead either to a total or a partial absence of sperm binding to the ZP [1, 2] or the absence of acrosome reaction [2, 3], or the defect of spermatozoon access to the perivitelline space and his attachment to the oelemma membrane.

Angiotensin converting enzyme (ACE) is a Zn²⁺ metalloprotease dipeptidyl carboxypeptidase widely distributed in mammalian organisms. It was first identified as a key component of the renin-angiotensin system (RAS) where its main function was to hydrolyse the inactive peptide, angiotensin I, into the physiologically active octapeptide angiotensin II. Angiotensin II plays an important role in controlling fluid electrolyte balance and systemic blood pressure [4–6]. ACE also inactivates the vasodilator peptide bradykinin by removal of its two coterminal dipeptides [7].

Two isoforms of ACE are known to exist in mammals. The larger protein, the somatic ACE (sACE) is expressed widely in the body. It is found in blood, vascular endothelial cells, epithelial cells of the proximal tubules of the kidney, brain, intestinal brush border [8, 9]. Epididymis, macrophages and testis Leydig cells also express this isozyme [10].

In contrast, the testicular or germinal isoform (tACE) is exclusively found in male germinal cells. In murine testis, tACE is found to be expressed in post-meiotic germinal cells during spermiogenesis [11] whereas in human testis, this protein is found in the golgi apparatus of spermatids at different stages of spermiogenesis. In ejaculated human spermatozoa, immunoelectron microscopy demonstrated that tACE is mainly located at the plasma membrane of the acrosomal region, equatorial segment, post acrosomal region and mid piece [12].

Both somatic and testicular ACE are encoded by the same gene consisting of 26 exons on chromosome 17q23 via two different tissue-specific promoters. Somatic ACE is transcribed from a promoter region upstream exon 1 in the 5′ region and is composed of a single polypeptide chain with two homologous domains, each one bearing an active catalytic site [7]. In contrast, tACE is transcribed from a promoter within intron 12 and it has only the active carboxyterminal domain of the sACE [13, 14].

Disruption of the mouse ACE gene has revealed the multiple physiological roles of ACE. Homozygous ACE−/− mice had lower blood pressure, atrophy of the renal cortex, thickened arteries and are unable to concentrate urine or maintain normal urinary salt balance. Besides, male ACE−/− mice sire few pups although they have normal testis structure and semen parameters (count, morphology and motility) [8, 15].

Transgenic mice lacking the somatic isoform but not the testicular one have the same renal and vascular abnormalities as those above. However, males’ sACE−/− are fertile [9, 16]. Moreover, angiotensinogen gene knockout male mice are fertile suggesting that this molecule is not the substrate of the tACE [8].

Transgenic male mice that express only the sACE but not the testicular one in the testis sire few pups despite mating normally [17].

The decreased fertility of these tACE−/− mice can be fully accounted for by the reduced capacity of their sperm to achieve fertilization in vivo as demonstrated by Hagaman et al. [16]. Indeed, despite normal functional parameters, very few spermatozoa from tACE−/− male mice are found in oviduct female regions 1 hr after mating compared to the wild mice. Thus, less than 5% of the eggs were fertilized in vivo from these tACE−/− mice matings versus 65% of the eggs from the wild mice matings [16]. The fertilized eggs by sperm from tACE−/− mice develop normally to the 8-cell stage in vitro and these males sire occasional pups [16]. Besides, in an in vitro assay, sperm from tACE−/− mice show defects in binding to ZP [16]. Based on these findings in mice models, we hypothesized that some infertile men that have the same phenotype as the tACE−/− mice, i.e. normal semen parameters (number, motility and morphology) and a defect of their spermatozoa to bind to the ZP in an in vitro fertilization cycle, may have a tACE defect. First, we investigated the tACE expression in spermatozoa, and then we performed a DNA sequencing of the tACE gene.

From January 2004 until December 2005 patients were included in this prospective study at the department of Histology and Biology of Reproduction at Tenon Hospital.

The mean age of the case-subjects was 41.4 ± 7.5 years and the mean age of their female partners was 34.5 ± 4.5 years at the time of the screening. The mean age of the control-subjects and their female partners was respectively 40.8 ± 3.9 and 33.8 ± 3.75 years. The average number of oocytes retrieved was 9.44 ± 3.32. The average number of mature oocytes was 7.65 ± 4.7.

Characterization of tACE in the spermatozoa pellets

A total of 10 cases and 14 control-subjects’ samples were extracted and western blotted.

As shown in Figure 1, the two lanes correspond to the glycosylated and non-glycosylated forms of the tACE. This protein is approximately 100 kDa.

Three case-subjects (4, 5 and 6) had no tACE expression when the lanes were compared to the standard protein (pyruvate kinase lanes). However, one control-subject has also no tACE expression. There’s no significant difference between the case and the control-subjects. The power of the statistical analysis is 34% (α = 0.05).

In the present study, western-blot was performed on 10 case-subjects and 14 control-subjects. Three case-subjects and one control-subject had no tACE expression. There was no statistic difference between the two groups. The power of the statistical analysis is 34% (α = 0.05).

PCR was performed on 12 blood samples; no mutation was detected in the tACE DNA sequences.

When we observed the phenotypes of case-subjects (spermatozoa survival and number of spermatozoa bound to the ZP), we did not find any significant difference between case subjects without tACE expression and those who have normal tACE expression (Table 1).

We underwent this study in the optics to find a cause to certain cases of male infertility presenting a phenotype similar to that of tACE gene knockout mice models.

Many previous studies using tACE knockout mice provided further information about the function of tACE in male fertility. Krege et al. [15] demonstrated that homozygous ACE knockout male mice sire few pups despite mating normally whereas homozygous female mutants are fertile. Hagaman et al. [16] observed that expression of ACE in sperm is important for egg fertilization. Sperm lacking tACE are deficient in transport within the oviduct and in binding to ZP [16]. It is on these results that we established the criteria of selection of our case-subjects.

However, in the study of Hagaman et al. [16], the average number of spermatozoa of male homozygous ACE−/− mice counted in wild female mice oviducts one hour after mating was sharply lower than that of wild male mice counted in the same conditions [16]. Consequently, we can suppose that in our study, the inclusive criteria of our case subjects were too drastic. We should have included men with IVF failure and either a low semen survival rate (i.e: low motility post-capacitation in vitro) or a decrease of the percentage of rapid progressive motility (type a) [18] although relationship between this motility defect and the tACE seems difficult to establish. Indeed, only Shibahara et al. [20] found a negative correlation between the membrane tACE activity and semen motility. Because of our drastic inclusive criteria, only 10 case-subjects were included in this study and we did not find any significant difference. Moreover, the power of the statistical analysis was too low (34%) and is certainly due to the low number of the patients included. We should have between 45 and 50 case-patients included and the same number in the control-group with a power of 80% to have a significant difference.

ICSI overcame this condition of total fertilization failure for 8 case subjects. However, two patients have a total fertilization failure when undergoing ICSI, despite a sufficient number of mature oocytes. Only 3 mature oocytes for patient n°3 and 2 oocytes for patient n°8 could be interpreted. They all had a premature condensation of the sperm chromatin (PCC). This condition is due to a cytoplasmic immaturity of the eggs and leads to a lack of oocytes activation [19]. Thus, despite our drastic criteria, two couples have both a female and male factors as causes of total fertilization failure.

The implication of the tACE in male fertility is far from having clearly been clarified. Métayer et al. [21] found that ejaculated mouse sperm have no remaining ACE. Thus, the sperm physiological changes may not relate to a direct role of ACE in capacitation, acrosomal reaction, or sperm-oocyte interaction. They have demonstrated in the rat and mouse that the germ cell membrane-bound ACE is completely released under an active form as the sperm passed through the proximal caput of the epididymis. Nevertheless, Yamaguchi et al. [22] showed that tACE is localized exclusively on the head of sperm from the cauda epididymis suggesting that tACE is involved in sperm-ZP interaction. Some authors reported that GPIase activity of ACE is necessary for sperm to acquire ZP binding activity [23]. Kondoh et al. postulated that some GPI (Glycosyl Phosphatidyl Inositol) anchored proteins on the sperm surface might play a role in ZP interaction after cleavage or that tACE may expose a ZP binding factor by shedding GPI-anchored proteins that block binding [23]. However, Fuchs et al. [24] indicated that sperm ACE is unable to cleave GPI anchored proteins but plays its crucial role in binding to the ZP thanks to its dicarboxypeptidase activity.

Testicular ACE can thus play a physiological role in oocyte fertilization, but at which step? Motility post-capacitation explaining the difficulty of ascension in the female oviducts of spermatozoa ACE−/−? Spermatozoa-ZP interaction? Spermatozoa binding to the oelemma? The most constantly described abnormality is the defect of spermatozoa-ZP interaction.

According to our literature analysis, tACE plays a crucial role in fertilization in rodents as demonstrated by tACE knock-out mice models. We based our study on these rodent models to look for a lack of the expression of the tACE in the semen of patients carrying the same phenotype: a failure of fertilization during an IVF cycle with no spermatozoa bound to the ZP despite normal semen parameters. In western-blot, we did not find any significant difference between the case and the control patients. The PCR was performed on blood samples of 10 case-subjects and 14 control-ones. No mutation was detected in the tACE DNA sequences. It would be advisable for our study to enlarge the criteria of selection to include all the animal model phenotypes, particularly men with IVF failure and either a low semen survival rate (i.e: low motility post-capacitation) or a decrease of the percentage of rapid progressive motility (type a) [18]. Besides, a multicentric study will allow us to include a sufficient number of subjects to evidence a significantly statistical difference between the case and the control subjects. Other techniques should be applied such as proteomic mapping, spectrophotometry or micro-array. ICSI offers a particularly effective therapeutic solution to these cases of male infertility but does not tackle the mechanisms in cause.