Fig. 4
Expression of TSGA10 in the patients’ spermatozoa. A Real-time quantitative PCR analysis of TSGA10 mRNA expression in semen samples from the patient and control. β-Actin was used as an internal control. B Western blotting analysis of the protein level of TSGA10 in the patient and normal control. α-tubulin was used as a loading control (C) Representative image of spermatozoa from fertile control and patients carrying the TSGA10 variant stained with an anti-TSGA10 antibody (red), an anti-α-tubulin antibody (green), and Hoechst (blue, nuclear marker). The TSGA10 signal was not detected in the sperm midpiece from the patient. Scale bar: 10 μm