Table 1 Characteristics of designed primers

STL assay

Genes

Forward primer (5' –3')

Reverse primer (5'–3’)

Telomere

TelG: ACACTAAGGTTTGGGTTT

GGGTTTGGGTTTGGGTTAGTGT

TelC: TGTTAGGTATCCCTATCCC

TATCCCTATCCCTATCCCTAACA

Beta-globin

(Single-copy gene)

hbgu: CGGCGGCGGGCGGCGCG

GGCTGGGCGGcttcatccacgttcaccttg

hbgd: GCCCGGCCCGCCGCGCC

CGTCCCGCCGgaggagaagtctgccgtt

mRNA expression

Beclin1

CAAGATCCTGGACCGTGTCA

GGCACTTTCTGTGGACATCATC

AMPKa1(PRKAA1)

ACAGCCGAGAAGCAGAAACAC

TCATGTGTGCCAACCTTCACT

ULK1

CCGCGAGAAGCACGATTTG

AGTCATACAGGGCCACGATG

BAX

GAGCAGATTATGAAGACAGGGG

ACGGCGGCAATCATCCTC

BCL2

AGATTGATGGGATCGTTGCCT

AGTCTACTTGCTCTGTGATGTTGT

B2M (Reference gene)

AGATGAGTATGCCTGCCGTGT

TGCGACATCTTCAAACCTCCAT