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Fig. 3 | Basic and Clinical Andrology

Fig. 3

From: Male obesity is associated with sperm telomere shortening and aberrant mRNA expression of autophagy-related genes

Fig. 3

Assessing viability and apoptotic changes in sperm samples from individuals in the study groups utilizing annexin V-FITC/PI staining and flowcytometry. This figure describes the assessment of viability and apoptotic alterations in normal-weight individuals, as well as individuals with obesity. The Annexin V-FITC assay is based on Annexin V coupled with the fluorescent dye, FITC, as a probe for the detection of phosphatidylserine in the outer layer of the apoptotic cell membrane. PI is used to assess permeability of cell membranes. (Q1: Necrosis (%), Q2: Late Apoptosis (%), Q3: Early Apoptosis (%), and Q4: Live Cells (%)). Abbreviations: PI; Propidium Iodide, FITC Fluorescein isothiocyanate

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Basic and Clinical Andrology

ISSN: 2051-4190