Fig. 8

Skin-derived precursors Schwann cells enhanced chemotaxis of M0 macrophages and secreted chemokines. A Induction and identification of M0 macrophages. Flow cytometry showed M0 macrophages expressed more CD11b after differentiated from THP-1 cells. B, C ELISA assay of MCP-1 and Col VI levels of different conditioned medium. D M0 macrophages chemotaxis under the condition of different conditioned medium visualized by crystal violet. E Qualification of cells that migrated. All values are represented as the mean ± standard deviation from three independent experiments, each with three replicates. *p < 0.05, ***p < 0.001; the student’s unpaired t-test was used for two-group comparisons; multiple-group comparisons were carried out by one-way ANOVA followed by the S–N-K test. ELISA: enzyme-linked immunosorbent assay; MCP-1: monocyte chemotactic protein 1; Col VI: Collagen VI