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Table 2 BLAST analysis results of amino acid sequences of positive phage clones

From: Screening of sperm antigen epitopes by phage display technique and its preliminary clinical application

Clone (No.)

Sequence

Homology

1

VDTVWTVKMGRV

FAM82C protein (6/12); cerebral protein-10 (6/12); NDUFA6 (6/12); sperm protein SSP411 and Spermatogenesis associated 20 (5/12)

4

EHPRLVGNPVKS

additional sex combs like 2 isoform 4 (10/12); selectin-like protein (6/12); polydom isoform 6 (6/12); RP11-427L11.3 (6/12)

5

DGHLLTSRFLLH

FLJ00025 protein (6/12); Intraflagellar transport 122 homolog (Chlamydomonas) (6/12); WDR10p (6/12); ZNF287 protein (6/12)

16

LGHVIFSTSIVH

hypothetical protein LOC9703 (8/12); KIF27C (7/12); intestinal mucin (6/12); MUC3B mucin (6/12)

18

TVPKETTPLTLT

tRNA selenocysteine associated protein (7/12); chromosome 6 open reading frame 52 (7/12); neural cell adhesion protein (7/12); C6orf52 protein (7/12)

25

DGDPRGPDSHQI

SCRL protein (7/12); THEG protein (6/12); Theg homolog isoform 2 (6/12); Granzyme A (6/12)

27

STQPAPVTIEQG

KIAA1324 protein (8/12); LIM domain only 4 (6/12); DNA-binding protein B (7/12); expressed in hematopoietic cells, heart, liver (6/12)

29

CNMTYKIYALHD

zinc finger, FYVE domain containing 26 (7/12); Leukocyte common antigen precursor (6/12); protein tyrosine phosphatase(6/12); T200 leukocyte common antigen precursor (6/12)

32

VPIKLNLYRSVH

chromosome 16 open reading frame 7 (7/12); ankyrin repeat-containing protein (6/12); nebulin (6/12); ZFP28 protein (7/12)

34

RILATPVSALAV

Bile acid Coenzyme A: amino acid N-acyltransferase (glycine N-choloyltransferase) (7/12); BAAT (7/12); hormonally upregulated Neu-associated kinase (8/12); Trinucleotide repeat containing 4 (7/12)

35

ARAPSSSAFSLL

Ig heavy chain variable region (8/12); circulating B cell antibody heavy chain variable region (8/12); UDP-glucuronosyltransferase (10/12); inner centromere protein (8/12)

54

ATVPPVGQVRVL

ZNF574 protein (7/12); putative doublecortin domain-containing protein (6/12); human immunodeficiency virus type I enhancer binding protein (7/12); Epididymal-specific lipocalin-10 precursor (6/12)

56

WQLPHHLSKHLL

ZNF206 protein (7/12); zinc finger protein 237 (8/12); Signal peptide peptidase-like 2B (7/12); PES1 (6/12)

57

SVADKLALVRPP

histone deacetylase 10 (7/12); OTTHUMP00000042163 (7/12); ZNF672 protein (8/12); Monocarboxylate transporter 7 (6/12)

58

YTMQSSGISRWQ

choroideremia-like protein (6/12); OATP-I (6/12); zinc finger protein 639 (6/12); retinoblastoma-associated factor 600 (6/12)

59

SSYTPIQMPYNT

tRNA isopentenyl transferase (7/12); Chromosome 10 open reading frame 130 (6/12); metallophosphoesterase domain containing 1 (6/12); OTTHUMP00000028881 (6/12)

  1. The DNA of sixteen positive phage clones was extracted and amplified by PCR, and PCR products were performed sequencing. The DNA sequences obtained were translated into amino acid sequences and subjected to Blast (http://blast.ncbi.nlm.nih.gov/Blast) in a non-redundant protein database to analyze their homology. Among these homologous sequences, the sequences of #1, #16, #25, and #54 clones had high homology with male reproductive system related proteins. Five amino acids (4–8) of #1 clone were identical to that of sperm protein SSP411 (418–422). The peptide sequence of #16 clone was similar to that of KIF27C. The peptide sequence of #25 clone was homologous with those of SCRL and THEG proteins. Six amino acids of #54 clone were the same as those of epididymal specific lipocalin-10 and putative doublecortin domain-containing protein