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Table 1 RNA sequencing and GeneChip (Affymetrix) data analysis of heat shock factor, heat shock protein and endoplasmic reticulum stress genes. FC; the log-fold changes, FDR; false discovery rate, RPKM; median expression values in reads per kilobase per million for thermosensitive genes before and after GnRHa treatment

From: Temperature is not a major factor in the differentiation of gonocytes into ad spermatogonia and fertility outcome in congenitally cryptorchid boys

Gene HIR/LIR
(RPKM)
log2FC / FDR before/after GnRHa
treatment
(RPKM)
log2FC / FDR Affymetrix probeset identifier
(highest variance probe set per gene)
Descended / undescended
(HIR + LIR, median log2 signal)
log2FC / FDR
Heat-shock protein genes
HSP90AA1 71.2/102.3 −0.53/0.01 75.6/78.1 −0.63/0.01 211968_s_at 10.11/10.26 n.s.
HSPA1A (HSP70) 6.58/8.8 −0.40/0.01 7.63/8.66 n.s. 202581_at 8.0/7.4 n.s.
HSPA2 1.69/2.49 n.s. 2.6/2.25 −0.61/0.02 211538_s_at 5.33/5.43 n.s.
HSPA4 39.6/49.3 −0.28/0.03 44.5/34.5 −0.90/0.0005 208814_at 6.78/6.49 n.s.
HSPA4L 20.1/18.0 n.s. 18.15/13.82 −0.90/0.01 205543_at 6.55/7.14 −0.6/0.049
DNAJB1 (HSP40) 28.0/34.3 −1.99/0.0006 29.8/27.9 −0.69/0.003 200664_s_at 8.0/8.47 n.s.
DNAJB2 (HSP40) 10.9/11.4 n.s. 11.93/11.90 −0.51/0.02 202500_at 6.48/6.83 n.s.
Endoplasmic reticulum stress genes
ERN1 1.41/3.15 −0.54/0.02 1.72/3.23 n.s. 207061_at 4.35/4.27 n.s.
TXNRD1 17.4/19.1 n.s. 15.3/13.5 −0.79/0.0001 201266_at 8.91/9.03 n.s.
PRKAR2B 83/80 n.s. 76.05/53.0 −0.97/0.0006 203680_at 10.9/11.05 n.s.
CASP3 8.94/9.23 n.s. 8.53/7.7 −0.72/0.002 202763_at 6.71/6.91 n.s.
EIF2AK3 7.38/7.0 n.s. 6.84/6.55 −0.56/0.01 218696_at 7.37/7.64 n.s.
ATF1 13.1/12.2 n.s. 12.3/8.3 −0.91/0.0003 1565269_s_at 6.33/6.73 n.s.
ATF6 12.49/12.44 n.s. 12.76/10.66 −0.76/0.002 217550_at 7.25/7.4 n.s.
SKIV2L2 23.4/27.3 n.s. 23.71/18.3 −0.87/0.0005 1562142_at 3.28/2.95 n.s.
Heat-shock factor genes
HSF1 13/14 n.s. 14.5/20.9 n.s. 213756_s_at 4.04/4.26 n.s.
HSF2 16.6/24 −0.52/0.0002 16.6/16 −0.82/0.0009 211220_s_at 5.48/5.35 n.s.
HSF4 0.81/0.99 n.s. 0.88/1.34 n.s. 210977_s_at 6.01/6.06 n.s.
HSF5 0.37/1.0 −1.42/0.0005 0.49/1.43 0.69/0.04 230718_at 3.86/3.46 n.s.
HSFY2 0.22/0.22 n.s. 0.17/0.83 1.77/0.0003 224007_at 3.06/3.14 n.s.
HSFX2 1.80/1.97 n.s. 2.1/2.91 n.s. 220314_at 5.41/5.24 n.s.