Table 1 RNA sequencing and GeneChip (Affymetrix) data analysis of heat shock factor, heat shock protein and endoplasmic reticulum stress genes. FC; the log-fold changes, FDR; false discovery rate, RPKM; median expression values in reads per kilobase per million for thermosensitive genes before and after GnRHa treatment
Gene | HIR/LIR (RPKM) | log2FC / FDR | before/after GnRHa treatment (RPKM) | log2FC / FDR | Affymetrix probeset identifier (highest variance probe set per gene) | Descended / undescended (HIR + LIR, median log2 signal) | log2FC / FDR |
|---|---|---|---|---|---|---|---|
Heat-shock protein genes | |||||||
HSP90AA1 | 71.2/102.3 | −0.53/0.01 | 75.6/78.1 | −0.63/0.01 | 211968_s_at | 10.11/10.26 | n.s. |
HSPA1A (HSP70) | 6.58/8.8 | −0.40/0.01 | 7.63/8.66 | n.s. | 202581_at | 8.0/7.4 | n.s. |
HSPA2 | 1.69/2.49 | n.s. | 2.6/2.25 | −0.61/0.02 | 211538_s_at | 5.33/5.43 | n.s. |
HSPA4 | 39.6/49.3 | −0.28/0.03 | 44.5/34.5 | −0.90/0.0005 | 208814_at | 6.78/6.49 | n.s. |
HSPA4L | 20.1/18.0 | n.s. | 18.15/13.82 | −0.90/0.01 | 205543_at | 6.55/7.14 | −0.6/0.049 |
DNAJB1 (HSP40) | 28.0/34.3 | −1.99/0.0006 | 29.8/27.9 | −0.69/0.003 | 200664_s_at | 8.0/8.47 | n.s. |
DNAJB2 (HSP40) | 10.9/11.4 | n.s. | 11.93/11.90 | −0.51/0.02 | 202500_at | 6.48/6.83 | n.s. |
Endoplasmic reticulum stress genes | |||||||
ERN1 | 1.41/3.15 | −0.54/0.02 | 1.72/3.23 | n.s. | 207061_at | 4.35/4.27 | n.s. |
TXNRD1 | 17.4/19.1 | n.s. | 15.3/13.5 | −0.79/0.0001 | 201266_at | 8.91/9.03 | n.s. |
PRKAR2B | 83/80 | n.s. | 76.05/53.0 | −0.97/0.0006 | 203680_at | 10.9/11.05 | n.s. |
CASP3 | 8.94/9.23 | n.s. | 8.53/7.7 | −0.72/0.002 | 202763_at | 6.71/6.91 | n.s. |
EIF2AK3 | 7.38/7.0 | n.s. | 6.84/6.55 | −0.56/0.01 | 218696_at | 7.37/7.64 | n.s. |
ATF1 | 13.1/12.2 | n.s. | 12.3/8.3 | −0.91/0.0003 | 1565269_s_at | 6.33/6.73 | n.s. |
ATF6 | 12.49/12.44 | n.s. | 12.76/10.66 | −0.76/0.002 | 217550_at | 7.25/7.4 | n.s. |
SKIV2L2 | 23.4/27.3 | n.s. | 23.71/18.3 | −0.87/0.0005 | 1562142_at | 3.28/2.95 | n.s. |
Heat-shock factor genes | |||||||
HSF1 | 13/14 | n.s. | 14.5/20.9 | n.s. | 213756_s_at | 4.04/4.26 | n.s. |
HSF2 | 16.6/24 | −0.52/0.0002 | 16.6/16 | −0.82/0.0009 | 211220_s_at | 5.48/5.35 | n.s. |
HSF4 | 0.81/0.99 | n.s. | 0.88/1.34 | n.s. | 210977_s_at | 6.01/6.06 | n.s. |
HSF5 | 0.37/1.0 | −1.42/0.0005 | 0.49/1.43 | 0.69/0.04 | 230718_at | 3.86/3.46 | n.s. |
HSFY2 | 0.22/0.22 | n.s. | 0.17/0.83 | 1.77/0.0003 | 224007_at | 3.06/3.14 | n.s. |
HSFX2 | 1.80/1.97 | n.s. | 2.1/2.91 | n.s. | 220314_at | 5.41/5.24 | n.s. |