Rubus apetalus (Rosaceae) improves spermatozoa characteristics, antioxidant enzymes and fertility potential in unilateral cryptorchid rats

Alumeti Munyali, Désiré; Tetsatsi Momo, Aimé Césaire; Bonsou Fozin, Georges Romeo; Deeh Defo, Patrick Brice; Petnga Tchatat, Yannick; Lieunang, Boris; Watcho, Pierre

doi:10.1186/s12610-020-00107-3

Basic and Clinical Andrology

Table 1 Effects of different treatments on sperm parameters

From: Rubus apetalus (Rosaceae) improves spermatozoa characteristics, antioxidant enzymes and fertility potential in unilateral cryptorchid rats

Sperm parameters
Groups	Viability (%)		Sperm count (10⁶/mL)		Motility (%)		Normality (%)
Groups	Right testis	Left testis	Right testis	Left testis	Right testis	Left testis	Right testis	Left testis
2 weeks
N	92.03 ± 0.5	91.03 ± 0.6	178.62 ± 7.00	179.62 ± 8.00	60.9 ± 4.05	61.06 ± 4.65	70.50 ± 0.87	68.07 ± 0.94
SO	89.26 ± 0.57	88.16 ± 0.68	113.30 ± 11.75	114.50 ± 12.45	66.05 ± 2.59	67.55 ± 3.49	65.56 ± 0.96	64.16 ± 1.64
DW	0#, c,¤^,¤	93.50 ± 1.42	0#, c,¤^,¤	92.12 ± 27.29	0#, c,¤	44.37 ± 0.83	0#, c,¤^,¤	50.85 ± 2.28
VIT E	0#, c,¤^,¤	87.36 ± 2.47	0#, c,¤^,¤	133.75 ± 15.45	0#, c,¤^,¤	58.02 ± 7.49	0#, c,¤^,¤	59.7 ± 2.66^*
AE 12	0#, c,¤^¤	91.81 ± 1.60	0#, c,¤^,¤	111.75 ± 17.44	0#, c,¤^,¤	65.47 ± 5.85	0#, c,¤^,¤	70.95 ± 1.74^***
AE 60	0#, c,¤^,¤	91.90 ± 3.53	0#, c,¤^,¤	107.75 ± 13.58	0#, c,¤^,¤	72.08 ± 4.17^*	0#, c,¤^,¤	71.11 ± 1.61^***
ME 12	0#, c,¤^,¤	90.10 ± 1.50	0#, c,¤^,¤	61.12 ± 5.92	0#, c,¤^,¤	75.34 ± 5.75^*	0#, c,¤^,¤	62.69 ± 1.08^***
ME 60	0#, c,¤^,¤	98.90 ± 1.86	0#, c,¤^,¤	63.50 ± 4.93	0#, c,¤^,¤	84.01 ± 2.08^***	0#, c,¤^,¤	75.04 ± 1.33^***
4 weeks
N	93.14 ± 0.67	91.03 ± 0.67	156.62 ± 7.50	157.87 ± 7.41	70.9 ± 3.85	71.84 ± 1.42	{66.13 ± 0.87)	67.35 ± 0.72
SO	87.56 ± 0.46	88.16 ± 0.68	151.20 ± 9.75	153.12 ± 3.47	75.05 ± 2.35	77.43 ± 2.08	65.06 ± 1.22	65.46 ± 1.24
DW	0#, c,¤	81.34 ± 1.85	0#, c,¤	88.50 ± 10.87	0#, c,¤	65.49 ± 1.68	0#, c,¤	53.45 ± 1.29
VIT E	0#, c,¤	87.17 ± 0.70	0#, c,¤	108.38 ± 12.88	0#, c,¤	74.41 ± 1.38	0#, c,¤	64.8 ± 2.78^*
AE 12	0#, c,¤	84.95 ± 0.83	0#, c,¤	74.88 ± 9.92	0#, c,¤	72.91 ± 2.61]	0#, c,¤	72.54 ± 1.21^***
AE 60	0#, c,¤	86.02 ± 1.58	0#, c,¤	102.50 ± 7.23	0#, c,¤	82.93 ± 3.06^**	0#, c,¤	78.54 ± 1.82^***
ME 12	0#, c,¤	84.01 ± 0.91	0#, c,¤	135.13 ± 24.95^*,Δ	0#, c,¤	80.39 ± 2.90^*	0#, c,¤^{, ¤}	51.71 ± 1.91^***
ME 60	0#, c,¤	91.34 ± 4.16^**	0#, c,¤	144.00 ± 28.80^*,Δ	0#, c,¤	85.67 ± 1.76^***	0#, c,¤	79.84 ± 1.41^***
8 weeks
N	92.12 ± 0.65	84.47 ± 2.94	184.65 ± 7.45	183 ± 7.29	84.47 ± 2.94	84.47 ± 2.94	67.94 ± 0.94	66.32 ± 0.81
SO	89.25 ± 0.75	89.83 ± 1.76	86.92 ± 3.86	185 ± 3.96	89.83 ± 1.76	89.83 ± 1.76	63.81 ± 0.87	64.16 ± 1.64
DW	0#, c,¤	72.92 ± 2.62	0#, c,¤	106.66 ± 6.08	0#, c,¤	72.92 ± 2.61	0#, c,¤	50.85 ± 2.28
VIT E	0#, c,¤	81.97 ± 3.05	0#, c,¤	150.62 ± 7.67^*	0#, c,¤	81.97 ± 3.05	0#, c,¤	59.7 ± 2.66^*
AE 60	0#, c,¤	85.59 ± 3.96^*	0#, c,¤	176.35 ± 4.03^{**, Δ,}•	0#, c,¤	85.59 ± 3.96^*	0#, c,¤	77.20 ± 2.62^***
ME 60	0#, c,¤	86.54 ± 2.37^*	0#, c,¤	147.50 ± 5.90^**,Δ,•	0#, c,¤	86.54 ± 2.37^*	0#, c,¤	79.75 ± 1.33^***

All values are expressed as mean ± SEM. Number of rat per group = 5
N normal, SO sham operated, DW distilled water, VIT E vitamin E, AE aqueous extract, ME methanolic extract
#: p < 0.001 compared to normal; c: p < 0.001 compared to sham operated; *p<0.05;**p<0.01;***p<0.001 compared to DW; ¤: p < 0.001 compared to left testis (same group); Δp < 0.001 compared to week 2 (same group); •p < 0.001 compared to week 4 (same group)

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ISSN: 2051-4190

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