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Table 1 Effects of in vitro culture and freezing on sperm parameters

From: Impact on ICSI outcomes of adding 24 h of in vitro culture before testicular sperm freezing: a retrospective study

Parameters Average (%) ± SDa p value b n
Effect of freezing (difference after thawing-before freezing)    
 Motility D0c −6.0 ± 13.7 0.0003 40
  Average motility after thawing D0 1.9 ± 4.8   
  Average motility before freezing D0 7.9 ± 14.7   
 Motility D1d −6.3 ± 9.9 0.0003 43
  Average motility after thawing D1 3.4 ± 7.6   
  Average motility before freezing D1 9.7 ± 12.7   
 Viability D0 −25.2 ± 18.3 <0.0001 40
  Average viability after thawing D0 54.4 ± 16.5   
  Average viability before freezing D0 79.6 ± 11.9   
 Viability D1 −20.8 ± 15.6 <0.0001 33
  Average viability after thawing D1 51.0 ± 18.9   
  Average viability before freezing D1 71.8 ± 13.7   
Effect of in vitro culture (difference after in vitro culture-before in vitro culture)    
 Motility +2.8 ± 12.0 0.0161 47
  Average motility after in vitro culture D1 before freezing 10.7 ± 13.55   
  Average motility before in vitro culture D0 before freezing 7.9 ± 14.6   
 Viability −8.3 ± 13.8 0.0070 35
  Average viability after in vitro culture D1 before freezing 71.0 ± 13.7   
  Average viability before in vitro culture D0 before freezing 79.3 ± 12.4   
Effect of freezing and in vitro culture (difference D1-D0 after thawing)    
 Motility +1.4 ± 5.4 0.0895 44
  Average motility after thawing D0 2.0 ± 4.9   
  Average motility after thawing D1 3.4 ± 7.6   
 Viability −2.0 ± 21.6 0.7201 34
  Average viability after thawing D0 54.0 ± 16.8   
  Average viability after thawing D1 52.0 ± 17.7   
  1. No Number of
  2. aSD: Standard Deviation
  3. bn: number of available data
  4. cD0: Day of the biopsy
  5. dD1: 24H after biopsy