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Analyse du statut oxydatif spermatique chez des patients infertiles

Analysis of sperm oxidative status in infertile patients

Basic and Clinical Andrology volume 22, pages 233–240 (2012)Cite this article

Résumé

Introduction

L’infertilité masculine constitue un problème de santé publique. Plusieurs facteurs sont à l’origine de ce phénomène. Actuellement, le stress oxydatif est incriminé comme l’une des principales causes. Dans notre étude, nous avons cherché une corrélation entre les marqueurs du stress oxydatif et les caractéristiques spermatiques (numération, mobilité, morphologie).

Matériel et méthodes

Nous avons évalué le statut oxydatif spermatique de 129 sujets infertiles. Ces sujets sont caractérisés par une infertilité de durée variable. Ils ont été subdivisés en quatre groupes: des sujets normozoospermiques considérés comme témoins (n=34); des asthénozoospermiques (Asthéno, n=43); des oligozoospermiques (Oligo, n=22) et tératozoospermiques (Térato, n=30). Parmi les marqueurs du stress oxydatif, nous avons évalué, dans le plasma séminal, le zinc, le calcium, le magnésium et le sélénium par spectrométrie d’absorption atomique à flamme et à four. Le malondialdéhyde (MDA) est dosé par spectrofluorométrie.

Résultats

Les résultats de notre étude montrent que les concentrations séminales du zinc et du sélénium sont plus élevées chez les normozoospermiques que les concentrations de ces mêmes éléments chez les autres groupes. La concentration séminale en zinc est significativement corrélée avec la numération spermatique (r=0,49; p<0,001) et le MDA (r=−0,35; p<0,05). La mobilité des spermatozoïdes est corrélée avec le calcium (r=0,41; p<0,001) et le magnésium (r=0,31; p<0,05). La concentration du MDA est plus élevée chez les trois groupes de patients: oligospermiques (3,22±1,37 μg/ml), asthénospermiques (3,52±1,93 μg/ml) et tératospermiques (3,64±1,73 μg/ml) par rapport aux témoins (2,32±0,94 μg/ml). Une seule corrélation positive a été observée entre le MDA et la morphologie (r=0,19; p<0,05).

Conclusion

Notre étude confirme que le stress oxydatif joue un rôle important dans le processus des altérations des spermatozoïdes. Les radicaux libres peuvent, en effet, modifier la structure membranaire ainsi que celle de l’acide désoxyribonucléique. Ces altérations conduisent aussi à une augmentation du pourcentage de spermatozoïdes de formes anormales.

Abstract

Introduction

Male infertility constitutes public health problems. Several factors are at the origin of this phenomenon. Currently, the oxidative stress is accused to be one of the leading causes. In our study, we sought a correlation between the markers of the oxidative stress and the sperm characteristics (morphology).

Material and methods

We evaluated the antioxidant status in the seminal plasma of 129 infertile men. Patients were characterized by infertility of variable duration. They were divided into four groups: normozoospermics who were considered as controls (n=34), asthenozoospermics (Astheno; n=43), oligozoospermics (Oligo; n=22) and teratozoospermics (Terato; n=30). Among the oxidative stress markers, we evaluated, in seminal plasma, zinc, calcium, magnesium and selenium by spectrophotometry of atomic absorption to flame and furnace. The malondialdehyde (MDA) is proportioned by spectrofluorometry.

Results

Our results show that the seminal concentrations of zinc and selenium are higher in the control group than the concentrations of these same elements in the three other groups. The seminal zinc concentration was significantly correlated with the sperm count (r=0.49; p < 0.001) and MDA (r=- 0.35; p<0.05). Sperm motility was correlated with calcium (r=0.41; p<0.001) and magnesium (r=0.31; p<0.05). The MDA concentration is higher in the three groups of patients: oligozoospermics (3.22±1.37 μg/ml), asthenozoospermics (3.52±1.93 μg/ml) and teratozoospermics (3.64±1.73 μg/ml) compared with controls (2.32±0.94 μg/ml). A single positive correlation was observed between the MDA and morphology (r=0.19; p<0.05).

Conclusion

Our study confirms that the oxidative stress plays an important role in the process of deteriorations of the spermatozoa. The free radicals can, indeed, modify the membrane structure as well as the membrane structure of the deoxyribonucleic acid. These deteriorations also lead to an increase in the percentage of sperm of abnormal forms.

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Authors and Affiliations

  1. Laboratoire de cytogénétique, génétique moléculaire et biologie de la reproduction humaines, CHU Farhat Hached Sousse, Sousse, Tunisie

    H. Ben Ali, A. Saad & M. Ajina

  2. Unité de médecine de la reproduction, CHU Farhat Hached de Sousse, Sousse, Tunisie

    F. Atig, S. Mehri, A. Saad & M. Ajina

Authors
  1. H. Ben Ali
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  2. F. Atig
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  3. S. Mehri
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  4. A. Saad
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  5. M. Ajina
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Correspondence to H. Ben Ali.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ben Ali, H., Atig, F., Mehri, S. et al. Analyse du statut oxydatif spermatique chez des patients infertiles. Basic Clin. Androl. 22, 233–240 (2012). https://doi.org/10.1007/s12610-012-0198-8

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  • DOI: https://doi.org/10.1007/s12610-012-0198-8

Mots clés

  • Infertilité
  • Stress oxydatif
  • Éléments trace

Keywords

  • Infertility
  • Oxidative stress
  • Trace elements

Basic and Clinical Andrology

ISSN: 2051-4190