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De la nature des vacuoles spermatiques aux résultats et indications de l’IMSI (intracytoplasmic morphologically selected sperm injection)

Nature of sperm-head vacuoles and results and indications of IMSI (intracytoplasmic morphologically selected sperm injection)

Résumé

En MSOME (high-magnification motile sperm organelle morphology examination), certains spermatozoïdes jugés normaux au grossissement × 400 ou × 200 peuvent présenter des vacuoles céphaliques. Si la large vacuole est décrite comme une empreinte de pouce nucléaire, la nature des vacuoles pourrait dépendre de leur taille, du nombre, de leur localisation et de leur profondeur. Cette revue de la littérature débat de la nature des vacuoles, analyse les résultats obtenus en IMSI (intracytoplasmic morphologically selected sperm injection) et en liste les indications.

Abstract

MSOME (high-magnification motile sperm organelle morphology examination) has shown that some spermatozoa, which appear morphologically normal when viewed at × 400 or × 200, present defects such as cephalic vacuoles when viewed under high magnification. The large vacuole is described as a nuclear thumbprint, but the nature may depend on the size, number, position and depth of the vacuole. This review summarizes the data available on the nature of vacuoles, analyzes the results of IMSI (intracytoplasmic morphologically selected sperm injection) and lists its potential indications.

Références

  1. 1.

    Gilbert SF (2007) La structure des gamètes. In: De Boeck (ed) Biologie du développement. 2e édition, Paris, chapitre 7

  2. 2.

    World Health Organization (2011) Reproductive health indicators for global monitoring: report of the second interagency meeting, Geneva. World Health Organization

  3. 3.

    Palermo G, Joris H, Devroey P, et al (1992) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340:17–18

    PubMed  CAS  Article  Google Scholar 

  4. 4.

    Loutradi KE, Tarlatzis BC, Goulis DG, et al (2006) The effects of sperm quality on embryo development after intracytoplasmic sperm injection. J Assist Reprod Genet 23:69–74

    PubMed  PubMed Central  Article  Google Scholar 

  5. 5.

    De Vos A, Van de Velde H, Joris H, et al (2003) Influence of individual sperm morphology on fertilization, embryo morphology, and pregnancy outcome of intracytoplasmic sperm injection. Fertil Steril 79:42–48

    PubMed  Article  Google Scholar 

  6. 6.

    Hotaling JM, Smith JF, Rosen M, et al (2011) The relationship between isolated teratozoospermia and clinical pregnancy after in vitro fertilization with or without intracytoplasmic sperm injection: a systematic review and meta-analysis. Fertil Steril 95:1141–1145

    PubMed  Article  Google Scholar 

  7. 7.

    Boitrelle F, Marchetti P, Mitchell V, et al (2011) Explorations fonctionnelles spécialisées du sperme et AMP. In: Poncelet C, Sifer C (eds) Physiologie, pathologie et thérapie de la reproduction chez l’humain. Springer, Paris, chapitre 30

    Google Scholar 

  8. 8.

    Fischbein A, Zabludovsky N, Eltes F, et al (1997) Ultramorphological sperm characteristics in the risk assessment of health effects after radiation exposure among salvage workers in Chernobyl. Environ Health Perspect 105:1445–1449

    PubMed  PubMed Central  Article  Google Scholar 

  9. 9.

    Bartoov B, Berkovitz A, Eltes F (2001) Selection of spermatozoa with normal nuclei to improve the pregnancy rate with intracytoplasmic sperm injection. N Engl J Med 345:1067–1068

    PubMed  CAS  Article  Google Scholar 

  10. 10.

    Bartoov B, Berkovitz A, Eltes F, et al (2002) Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl 23:1–8

    PubMed  Google Scholar 

  11. 11.

    Sermondade N, Sifer C (2011) Motile sperm organelle morphology examination: Toward a consensus? Gynecol Obstet Fertil 39:309–314

    PubMed  CAS  Article  Google Scholar 

  12. 12.

    Berkovitz A, Eltes F, Yaari S, et al (2005) The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod 20:185–190

    PubMed  Article  Google Scholar 

  13. 13.

    Berkovitz A, Eltes F, Ellenbogen A, et al (2006) Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome? Hum Reprod 21:1787–1790

    PubMed  Article  Google Scholar 

  14. 14.

    Vanderzwalmen P, Hiemer A, Rubner P, et al (2008) Blastocyst development after sperm selection at high magnification is associated with size and number of nuclear vacuoles. Reprod Biomed Online 17:617–627

    PubMed  Article  Google Scholar 

  15. 15.

    Cassuto NG, Bouret D, Plouchart JM, et al (2009) A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality. Fertil Steril 92:1616–1625

    PubMed  Article  Google Scholar 

  16. 16.

    Peer S, Eltes F, Berkovitz A, et al (2007) Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37 °C? Fertil Steril 88:1589–1594

    PubMed  Article  Google Scholar 

  17. 17.

    Franco JG, Baruffi RL, Mauri AL, et al (2008) Significance of large nuclear vacuoles in human spermatozoa: implications for ICSI. Reprod Biomed Online 17:42–45

    PubMed  Article  Google Scholar 

  18. 18.

    Franco Jr JG, Mauri AL, Petersen CG, et al (2011) Large nuclear vacuoles are indicative of abnormal chromatin packaging in human spermatozoa. Int J Androl (sous presse)

  19. 19.

    Garolla A, Fortini D, Menegazzo M, et al (2008) High-power microscopy for selecting spermatozoa for ICSI by physiological status. Reprod Biomed Online 17:610–616

    PubMed  Article  Google Scholar 

  20. 20.

    Travers A, Perdrix A, Legrand F, et al (2009) Les larges vacuoles des têtes spermatiques sont-elles associées à des altérations du noyau ou de l’acrosome du spermatozoïde ? Andrologie 20:247–256

    Article  Google Scholar 

  21. 21.

    Perdrix A, Travers A, Chelli MH, et al (2011) Assessment of acrosome and nuclear abnormalities in human spermatozoa with large vacuoles. Hum Reprod 26:47–58

    PubMed  CAS  Article  Google Scholar 

  22. 22.

    Boitrelle F, Ferfouri F, Petit JM, et al (2011) Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod 26:1650–1658

    PubMed  CAS  Article  Google Scholar 

  23. 23.

    Watanabe S, Tanaka A, Fujii S, et al (2011) An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay. Hum Reprod 26:978–986

    PubMed  CAS  Article  Google Scholar 

  24. 24.

    Kacem O, Sifer C, Barraud-Lange V, et al (2010) Sperm nuclear vacuoles, as assessed by motile sperm organellar morphological examination, are mostly of acrosomal origin. Reprod Biomed Online 20:132–137

    PubMed  CAS  Article  Google Scholar 

  25. 25.

    Zamboni L, Zemjanis R, Stefanini M (1971) The fine structure of monkey and human spermatozoa. Anat Rec 169:129–153

    PubMed  CAS  Article  Google Scholar 

  26. 26.

    Hammoud SS, Nix DA, Zhang H, et al (2009) Distinctive chromatin in human sperm packages genes for embryo development. Nature 460:473–478

    PubMed  CAS  PubMed Central  Google Scholar 

  27. 27.

    Bartoov B, Berkovitz A, Eltes F, et al (2003) Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril 80:1413–1419

    PubMed  Article  Google Scholar 

  28. 28.

    Hazout A, Dumont-Hassan M, Junca AM, et al (2006) Highmagnification ICSI overcomes paternal effect resistant to conventional ICSI. Reprod Biomed Online 12:19–25

    PubMed  Article  Google Scholar 

  29. 29.

    Antinori M, Licata E, Dani G, et al (2008) Intracytoplasmic morphologically selected sperm injection: a prospective randomized trial. Reprod Biomed online 16:835–841

    PubMed  Article  Google Scholar 

  30. 30.

    Nadalini M, Tarozzi N, Distratis V, et al (2009) Impact of intracytoplasmic morphologically selected sperm injection on assisted reproduction outcome: a review. Reprod Biomed Online 19:45–55

    PubMed  Article  Google Scholar 

  31. 31.

    Souza Setti A, Ferreira RC, Paes de Almeida Ferreira Braga D, et al (2010) Intracytoplasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod Biomed Online 21:450–455

    PubMed  Article  Google Scholar 

  32. 32.

    Junca AM, Cohen-Bacrie P, Belloc S, et al (2009) Teratozoospermia at the time of intracytoplasmic morphologically selected sperm injection (IMSI). Gynecol Obstet Fertil. 37:552–557

    PubMed  Article  Google Scholar 

  33. 33.

    Dumont M, Junca AM, Belloc S, et al (2011) L’utilisation du MSOME: expérience de six ans. Andrologie 21:83–89

    Article  Google Scholar 

  34. 34.

    Balaban B, Yakin K, Alatas C, et al (2011) Clinical outcome of intracytoplasmic injection of spermatozoa morphologically selected under high magnification: a prospective randomized study. Reprod Biomed Online (sous presse)

  35. 35.

    Wilding M, Coppola G, di Matteo L, et al (2011) Intracytoplasmic injection of morphologically selected spermatozoa (IMSI) improves outcome after assisted reproduction by deselecting physiologically poor quality spermatozoa. J Assist Reprod Genet 28:253–62

    PubMed  PubMed Central  Article  Google Scholar 

  36. 36.

    Hammoud I, Albert M, Bergere M, et al (2009) Can IMSI (× 6 000) be more efficient than classical ICSI selecting spermatozoa without nuclear fragmentation? Abstracts du 25e Congrès de l’ESHRE, Amsterdam

    Google Scholar 

  37. 37.

    Ai L, Liu SY, Huang J, et al (2010) Intracytoplasmic morphologically selected sperm injection of testicular sperm: clinical outcome in azoospermia patients. Zhonghua Nan Ke Xue 16: 826–829

    PubMed  Google Scholar 

  38. 38.

    Berkovitz A, Eltes F, Paul M, et al (2007) The chance of having a healthy normal child following intracytoplasmic morphologicallyselected sperm injection (IMSI) treatment is higher compared to conventional IVF-ICSI treatment. Fertil Steril 88:20

    Article  Google Scholar 

  39. 39.

    Vanderzwalmen P, Fallet C (2010) IMSI: indications, results and reflexions. J Gynecol Obstet Biol Reprod 39:22–25

    CAS  Article  Google Scholar 

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Correspondence to F. Boitrelle.

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Boitrelle, F., Ferfouri, F., Salama, S. et al. De la nature des vacuoles spermatiques aux résultats et indications de l’IMSI (intracytoplasmic morphologically selected sperm injection). Basic Clin. Androl. 21, 234–239 (2011). https://doi.org/10.1007/s12610-011-0147-y

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Mots clés

  • Vacuole spermatique
  • MSOME
  • IMSI
  • Indications et résultats

Keywords

  • Sperm vacuole
  • MSOME
  • IMSI
  • Indications and results