Skip to main content
Download PDF

Apport de l’exploration cytogénétique et ultrastructurale dans le pronostic de fertilité des sujets globozoospermiques

Contributions of cytogenetic and ultrastructural exploration in fertility prognosis for subjects with globozoospermia

Basic and Clinical Andrology volume 21, pages 240–246 (2011)Cite this article

Résumé

La globozoospermie est une forme sévère de tératozoospermie caractérisée par des spermatozoïdes à tête ronde avec absence ou présence d’un acrosome rudimentaire. L’objectif de cette étude est d’analyser les spermatozoïdes de six patients présentant un syndrome de globozoospermie et de rendre compte de l’inefficacité de plusieurs tentatives de fécondation in vitro avec ICSI. L’exploration de ces sujets a été réalisée par l’étude du taux d’aneuploïdies spermatiques par hybridation in situ fluorescente (sperm-FISH) pour les chromosomes X, Y et 18, du taux de fragmentation d’ADN par la technique TUNEL ainsi que par l’étude ultrastructurale détaillée de la morphologie spermatique en utilisant la microscopie électronique à transmission (MET). Onze tentatives d’ICSI ont été réalisées chez ces patients, avec un taux de fécondation faible (9,37 %), et aucune grossesse n’a été obtenue. Cette étude a permis de confirmer la variabilité des phénotypes spermatiques observés dans ce syndrome et de faible taux de fécondation après FIV-ICSI, et ce, quel que soit le phénotype.

Abstract

Globozoospermia is a severe form of teratozoospermia characterized by round-headed sperms with absence or presence of a rudimentary acrosome. The objective of this study is to analyze sperm from six patients with globozoospermia syndrome and report the results of 11 intracytoplasmic sperm injection (ICSI) attempts. The investigation of these issues was carried out by studying the sperm aneuploidy rate by fluorescent in situ hybridization (sperm-FISH) for chromosomes X, Yand 18. The rate of DNA fragmentation was studied by using the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) technique and a detailed ultrastructural morphology study of the sperm using transmission electron microscopy. Eleven ICSI attempts were performed in patients with low fertilization rate, (9.37%) and pregnancy did not occur. This study confirmed the variability of sperm phenotypes observed in this syndrome and the low fertilization rates after IVF-ICSI regardless of the phenotype.

Références

  1. Schirren CG, Holstein AF, Schirren C (1971) Uber die Morphogenese rundkopfiger Spermatozoen des Menschen. Andrologie 3:117–125

    Google Scholar 

  2. Aitken RJ, Kerr L, Bolton V, Hargreave T (1990) Analysis of sperm function in globozoospermia: implications for the mechanism of sperm-zona interaction. Fertil Steril 54:701–707

    PubMed  CAS  Google Scholar 

  3. Dam AH, Feenstra I, Westphal JR, et al (2007) Globozoospermia revisited. Hum Reprod Update 13:63–75

    Article  PubMed  CAS  Google Scholar 

  4. Holstein AF, Schirren CG, Schirren C, Mauss J (1973) Round headed spermatozoa: a cause of male infertility. Dtsch Med Wochenschr 98:61–62

    Article  PubMed  CAS  Google Scholar 

  5. Carrell DT, Wilcox AL, Udoff LC, et al (2001) Chromosome 15 aneuploidy in the sperm and conceptus of a sibling with variable familial expression of round-headed sperm syndrome. Fertil Steril 76:1258–1260

    Article  PubMed  CAS  Google Scholar 

  6. Carrell DT, Emery BR, Liu L (1999) Characterization of aneuploidy rates, protamine levels, ultrastructure, and functional ability of round-headed sperm from two siblings and implications for intracytoplasmic sperm injection. Fertil Steril 71:511–516

    Article  PubMed  CAS  Google Scholar 

  7. Kullander S, Rausing A (1975) On round-headed human spermatozoa. Int J Fertil 20:33–40

    PubMed  CAS  Google Scholar 

  8. Dam AH, Koscinski I, Kremer JA, et al (2007) Homozygous mutation in SPATA16 is associated with male infertility in human globozoospermia. Am J Hum Genet 81:813–820

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  9. Florke-Gerloff S, Topfer-Petersen E, Muller-Esterl W, et al (1984) Biochemical and genetic investigation of roundheaded spermatozoa in infertile men including two brothers and their father. Andrologia 16:187–202

    Article  PubMed  CAS  Google Scholar 

  10. Christensen GL, Ivanov IP, Atkins JF, et al (2006) Identification of polymorphisms in the Hrb, GOPC and Csnk2a2 genes in two men with globozoospermia. J Androl 27:11–15

    Article  PubMed  CAS  Google Scholar 

  11. Trokoudes KM, Danos N, Kalogirou L, et al (1995) Pregnancy with spermatozoa from a globozoospermic man after intracytoplasmic sperm injection treatment. Hum Reprod 10:880–882

    PubMed  CAS  Google Scholar 

  12. Harbuz R, Zouari R, Pierre V, et al (2011) A recurrent deletion of DPY19L2 causes infertility in man by blocking sperm head elongation and acrosome formation. Am J Hum Genet 88:351–361

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  13. World Health Organization (1999) Laboratory manual for the examination of human semen and sperm-cervical mucus interaction 4th ed. Cambridge University, Cambridge

    Google Scholar 

  14. Wheater, Young, Heat (2004) Histologie fonctionnelle. De Boeck Université 4e édition

  15. Kierszenbaum AL, Tres LL, Rivkin E (2003) Acroplaxome, an actin-keratin-containing plate, anchors the acrosome to the nucleus during shaping of the spermatid head. Mol Biol Cell 14:4628–4640

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  16. Kierszenbaum AL, Rivkin E, Tres LL (2003) The actin-based motor myosin Va is a component of the acroplaxome, an acrosome nuclear envelope junctional plate, and of manchetteassociated vesicles. Cytogenet Genome Res 103:337–344

    Article  PubMed  CAS  Google Scholar 

  17. Kierszenbaum AL, Tres LL, Rivkin E, et al (2004) The acroplaxome is a docking site of Golgi-derived myosin Va/Rab27a/bcontaining proacrosomal vesicles in wild type and Hrb mutant mouse spermatids. Biol Reprod 70:1400–1410

    Article  PubMed  CAS  Google Scholar 

  18. Kierszenbaum AL, Tres LL (2004) The acrosomeacroplaxome-manchette complex and the shaping of the spermatid head. Arch Histol Cytol 67:271–284

    Article  PubMed  CAS  Google Scholar 

  19. Schmiady H, Schulze W, Scheiber I, Pfüller B (2005) High rate of premature chromosome condensation in human oocytes following microinjection with round-headed sperm: case report. Hum Reprod 20:1319–1323

    Article  PubMed  Google Scholar 

  20. Heindryckx B, Van der Elst J, De Sutter P, Dhont M (2005) Treatment option for spermor oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI. Hum Reprod 20:2237–2241

    Article  PubMed  CAS  Google Scholar 

  21. Rybouchkin AV, Van der Straeten F, Quatacker J, et al (1997) Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity. Fertil Steril 68:1144–1147

    Article  PubMed  CAS  Google Scholar 

  22. Kim ST, Cha YB, Park JM, Gye MC (2001) Successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection using round-headed spermatozoa and assisted oocyte activation in a globozoospermic patient with mosaic down syndrome. Fertil Steril 75:445–447

    Article  PubMed  CAS  Google Scholar 

  23. Larson KL, Brannian JD, Singh NP, et al (2001) Chromatin structure in globozoospermia: a case report. J Androl 22:424–431

    PubMed  CAS  Google Scholar 

  24. Vicari E, Perdichizzi A, De Palma A, et al (2002) Globozoospermia is associated with chromatin structure abnormalities: case report. Hum Reprod 17:2128–2133

    Article  PubMed  Google Scholar 

  25. Achard V, Paulmyer-Lacroix O, Mercier G, et al (2007) Reproductive failure in patients with various percentage of macronuclear spermatozoa: high level of aneuploid and polyploid spermatozoa. J Androl 28:600–606

    Article  PubMed  Google Scholar 

  26. Ditzel N, El-Danasouri I, Just W, Sterzik K (2005) Higher aneuploidy rates of chromosomes 13, 16, and 21 in a patient with globozoospermia. Fertil Steril 84:217–218

    Article  PubMed  Google Scholar 

  27. Martin RH, Greene C, Rademaker AW (2003) Sperm chromosome aneuploidy analysis in a man with globozoospermie. Fertil Steril 79:1662–1664

    Article  PubMed  Google Scholar 

  28. Battaglia DE, Koehler JK, Klein NA, Tucker MJ (1997) Failure of oocyte activation after intracytoplasmic sperm injection using round-headed sperm. Fertil Steril 68:118–122

    Article  PubMed  CAS  Google Scholar 

  29. Vegni-talluri M, Menchini-Fabris F, Renieri T (1977) A possible haploid effect in acrosome malformations of human spermatozoa. Andrologia 9:315–322

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service de gynécologie obstétrique, laboratoire de biologie de la reproduction, centre de procréation médicalement assistée, hôpital Aziza-Othmana, place de la Kasbah, 1008, Tunis, Tunisie

    A. Zhioua, G. Merdassi, R. Bhouri & F. Zhioua

  2. Laboratoire commun de microscopie électronique, faculté de médecine de Tunis, Tunis, Tunisie

    A. Ben Ammar

  3. Laboratoire de cytogénétique, institut Pasteur de Tunis, Tunis, Tunisie

    A. Amouri

  4. Laboratoire d’histologie, embryologie, biologie de la reproduction, cytogénétique et génétique médicale, CHI de Poissy-Saint-Germain, F-78303, Poissy cedex, France

    F. Ferfouri & F. Vialard

  5. EA2493, UVSQ, Versailles, F-78000, France

    F. Ferfouri & F. Vialard

Authors
  1. A. Zhioua
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Merdassi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Bhouri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. Ferfouri
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Ben Ammar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Amouri
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. F. Vialard
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. F. Zhioua
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. Merdassi.

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Zhioua, A., Merdassi, G., Bhouri, R. et al. Apport de l’exploration cytogénétique et ultrastructurale dans le pronostic de fertilité des sujets globozoospermiques. Basic Clin. Androl. 21, 240–246 (2011). https://doi.org/10.1007/s12610-011-0145-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12610-011-0145-4

Mots clés

Keywords

Basic and Clinical Andrology

ISSN: 2051-4190