Riley FJ, Masters WH (1956) Problems of male fertility. III. Bacteriology of human semen. Fertil Steril 7:128–132
PubMed
CAS
Google Scholar
Auroux M (1984) Non-spermatozoal cells in human semen: a study of 1,243 subfertile and 253 fertile men. Arch Androl 12:197–201
Article
PubMed
CAS
Google Scholar
Comhaire F, Verschraegen G, Vermeulen L (1980) Diagnosis of accessory gland infection and its possible role in male infertility. Int J Androl 3:32–45
Article
PubMed
CAS
Google Scholar
Aitken RJ, Clarkson JS (1987) Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil 81:459–469
Article
PubMed
CAS
Google Scholar
Barratt CL, Bolton AE, Cooke ID (1990) Functional significance of white blood cells in the male and female reproductive tract. Hum Reprod 5:639–648
PubMed
CAS
Google Scholar
Jochum M, Pabst W, Schill WB (1986) Granulocyte elastase as a sensitive diagnostic parameter of silent male genital tract inflammation. Andrologia 18:413–419
Article
PubMed
CAS
Google Scholar
Satta A, Stivala A, Garozzo A, et al (2006) Experimental Chlamydia trachomatis infection causes apoptosis in human sperm. Hum Reprod 21:134–137
Article
PubMed
Google Scholar
Diaz-Garcia FJ, Herrera-Mendoza AP, Giono-Cerezo S, Guerra-Infanta FM (2006) Mycoplasma hominis attaches and locates intracellularly in human spermatozoa. Hum Reprod 21:1591–1598
Article
PubMed
Google Scholar
Villegas J, Schulz M, Soto L, Sanchez R (2005) Bacteria induce expression of apoptosis in human spermatozoa. Apoptosis 10:105–110
Article
PubMed
CAS
Google Scholar
Eggert-Kruse W, Rohr G, Kunt B, et al (2003) Prevalence of Chlamydia trachomatis in subfertile couples. Fertil Steril 80:660–663
Article
PubMed
Google Scholar
Gdoura R, Kchaou W, Ammar-Keskes L, et al (2008) Assessment of Chlamydia trachomatis, Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium in semen and first void urine specimens of asymptomatic male partners of infertile couples. J Androl 29:198–206
Article
PubMed
CAS
Google Scholar
Gallegos G, Ramos B, Santiso R, Goyanes V (2008) Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma. Fertil Steril 90:328–334
Article
PubMed
Google Scholar
Eggert-Kruse W, Rohr G, Demirakca T, et al (1997) Chlamydial serology in 1,303 asymptomatic subfertile couples. Hum Reprod 12:1464–1475
Article
PubMed
CAS
Google Scholar
Idahl A, Abramsson L, Kumlin U, et al (2007) Male serum Chlamydia trachomatis IgA and IgG, but not heat shock protein 60 IgG, correlates with negatively affected semen characteristics and lower pregnancy rates in the infertile couple. Int J Androl 30:99–107
Article
PubMed
CAS
Google Scholar
Joki-Korpela P, Sahrakorpi N, Halttunen M, et al (2008) The role of Chlamydia trachomatis infection in male infertility. Fertil Steril Aug 13 [Epub ahead of print]
Rodin DM, Larone D, Goldstein M (2003) Relationship between semen cultures, leukospermia, and semen analysis in men undergoing fertility evaluation. Fertil Steril 79(Suppl 3): 1555–1558
Article
PubMed
Google Scholar
Dejucq N, Jégou B (2001) Viruses in the mammalian male genital tract and their effects on the reproductive system. Microbiol Mol Biol Rev 65:208–231
Article
PubMed
CAS
PubMed Central
Google Scholar
Nicopoullos JD, Almeida PA, Ramsay JW, Gilling-Smith C (2004) The effect of human immunodeficiency virus on sperm parameters and the outcome of intrauterine insemination following sperm washing. Hum Reprod 19:2289–2297
Article
PubMed
Google Scholar
Garrido N, Meseguer M, Remohi J, et al (2005) Semen characteristics in human immunodeficiency virus (HIV) — and hepatitis C (HCV) — seropositive males: predictors of the success of viral removal after sperm washing. Hum Reprod 20:1028–1034
Article
PubMed
Google Scholar
Bezold G, Politch JA, Kiviat NB, et al (2007) Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia. Fertil Steril 87:1087–1097
Article
PubMed
PubMed Central
Google Scholar
Kapranos N, Petrakou E, Anastasiadou C, Kotronias D (2003) Detection of herpes simplex virus, cytomegalovirus, and Epstein-Barr virus in the semen of men attending an infertility clinic. Fertil Steril 79(Suppl 3):1566–1570
Article
PubMed
Google Scholar
Ricci G, Presani G, Guaschino S, et al (2000) Leukocyte detection in human semen using flow cytometry. Hum Reprod 15:1329–1337
Article
PubMed
CAS
Google Scholar
Sanocka D, Jedrzejczak P, Szumala-Kaekol A, et al (2003) Male genital tract inflammation: the role of selected interleukins in regulation of pro-oxidant and antioxidant enzymatic substances in seminal plasma. J Androl 24:448–455
Article
PubMed
Google Scholar
Henkel R, Kierspel E, Stalf T, et al (2005) Effect of reactive oxygen species produced by spermatozoa and leukocytes on sperm functions in non-leukocytospermic patients. Fertil Steril 83:635–642
Article
PubMed
CAS
Google Scholar
Trum JW, Mol BW, Pannekoek Y, et al (1998) Value of detecting leukocytospermia in the diagnosis of genital tract infection in subfertile men. Fertil Steril 70:315–319
Article
PubMed
CAS
Google Scholar
Keck C, Gerber-Schäfer C, Clad A, et al (1998) Seminal tract infections: impact on male fertility and treatment options. Hum Reprod Update 4:891–903
Article
PubMed
CAS
Google Scholar
Brinkmann V, Reichard U, Goosmann C, et al (2004) Neutrophil extracellular traps kill bacteria. Science 303:1532–1535
Article
PubMed
CAS
Google Scholar
Fuchs TA, Abed U, Goosmann C, et al (2007) Novel cell death program leads to neutrophil extracellular traps. J Cell Biol 176:231–241
Article
PubMed
CAS
PubMed Central
Google Scholar
Eggert-Kruse W, Kiefer I, Beck C, et al (2007) Role for tumor necrosis factor alpha (TNF-α) and interleukin 1-beta (IL-1beta) determination in seminal plasma during infertility investigation. Fertil Steril 87:810–823
Article
PubMed
CAS
Google Scholar
Zorn B, Virant-Klun I, Meden-Vrtovec H (2000) Semen granulocyte elastase: its relevance for the diagnosis and prognosis of silent genital tract inflammation. Hum Reprod 15:1978–1984
Article
PubMed
CAS
Google Scholar
Bourgeon F, Evrard B, Brillard-Bourdet M, et al (2004) Involvement of semenogelin-derived peptides in the antibacterial activity of human seminal plasma. Biol Reprod 70:768–774
Article
PubMed
CAS
Google Scholar
Linge HM, Collin M, Giwercman A, et al (2008) The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma. J Interferon Cytokine Res 28:191–196
Article
PubMed
CAS
Google Scholar
Rowe PJ, Comhaire FH, Hargreave TB, et al (2000) Objective criteria for diagnostic categories in the standardized management of male infertility: male accessory gland infection (MAGI). In: Rowe PJ, Comhaire FH, Hargreave TB, Mahmoud AMA (eds), WHO Manual for the Standardized Investigation, Diagnosis and Management of the Infertile Male. World Health Organization. Cambridge University Press, Cambridge, pp. 52–54
Google Scholar
Koppers AJ, De Iuliis GN, Finnie JM, et al (2008) Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa. J Clin Endocrinol Metab 93:3199–3207
Article
PubMed
CAS
Google Scholar
Burden HP, Holmes CH, Persad R, Whittington K (2005) Prostasomes their effects on human male reproduction and fertility. Hum Reprod Update 12:283–292
Article
PubMed
Google Scholar
Saez F, Motta C, Boucher D, Grizard G (2000) Prostasomes inhibit the NADPH-oxidase activity of human neutrophils. Mol Hum Reprod 6:883–891
Article
PubMed
CAS
Google Scholar
Vignon F, Clavert A, Cranz C, et al (1993) Alterations in the lipid composition of seminal plasma in patients with a chronic infection of the urogenital tract. Urol Int 50:36–38
Article
PubMed
CAS
Google Scholar
Zhou CX, Zhang YL, Xiao L, et al (2004) An epididymis-specific beta-defensin is important for the initiation of sperm maturation. Nat Cell Biol 6:458–464
Article
PubMed
CAS
Google Scholar
World Health Organization, WHO (1999) Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. 4th ed. Cambridge University Press, Cambridge
Google Scholar
Ziyyat A, Barraud-Lange V, Sifer C, et al (2008) Paradoxical increase of sperm motility and seminal carnitine associated with moderate leukocytospermia in infertile patients. Fertil Steril 90:2257–2263
Article
PubMed
Google Scholar
Elzanaty S, Richthoff J, Malm J, Giwercman A (2002) The impact of epididymal and accessory gland function on sperm motility. Hum Reprod 17:2904–2911
Article
PubMed
CAS
Google Scholar
Modrich RD, Maccioni M, Molina R, et al (2005) Reduced semen quality in chronic prostatitis patients that have cellular autoimmune response to prostate antigens. Hum Reprod 20:2567–2572
Article
Google Scholar
Alvarez JG, Sharma RK, Ollero M, et al (2002) Increased DNA damage in sperm from leukocytospermic semen samples as determined by the sperm chromatin structure assay. Fertil Steril 78:319–329
Article
PubMed
Google Scholar
Armstrong JS, Rajasekaran M, Chamulitrat W, et al (1999) Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radic Biol Med 26:869–880
Article
PubMed
CAS
Google Scholar
Wang X, Sharma RK, Gupta A, et al (2003) Alterations in mitochondria membrane potential and oxidative stress in infertile men: a prospective observational study. Fertil Steril 80(Suppl 2):844–850
Article
PubMed
Google Scholar
Perdichizzi A, Nicoletti A, La Vignera S, et al (2007) Effects of tumor necrosis factor alpha on human sperm motility and apoptosis. J Clin Immunol 27:152–162
Article
PubMed
CAS
Google Scholar
Krause W (2008) Male accessory gland infection. Andrologia 40:113–116
Article
PubMed
CAS
Google Scholar
Grunewald S, Said TM, Paasch U, et al (2008) Relationship between sperm apoptosis signaling and oocyte penetration capacity. Int J Androl 31:325–330
Article
PubMed
CAS
Google Scholar
Agarwal A, Allamaneni SSR, Nallella KP, et al (2005) Correlation of reactive oxygen species levels with the fertilization rate after in vitro fertilization: a qualified meta-analysis. Fertil Steril 84:228–231
Article
PubMed
CAS
Google Scholar
Jedrzejczak P, Fraczek M, Szumala-Kakol A, et al (2005) Consequences of semen inflammation on fertilization capacity of spermatozoa in in vitro conditions. Int J Androl 28:275–283
Article
PubMed
CAS
Google Scholar
Zorn B, Virant-Klun I, Vidmar G, et al (2004) Seminal elastase-inhibitor complex, a marker of genital tract inflammation, and negative IVF outcome measures: a role for a silent inflammation? Int J Androl 27:368–374
Article
PubMed
CAS
Google Scholar
Zorn B, Vidmar G, Meden-Vrtovec H (2003) Seminal reactive oxygen species as predictors of fertilization, embryo quality and pregnancy rates after conventional in vitro fertilization and intracytoplasmic sperm injection. Int J Androl 26:279–285
Article
PubMed
CAS
Google Scholar
Naber KG, Bergman B, Bishop MC, et al (2001) EAU guidelines for the management of urinary and male genital tract infections. Urinary Tract Infection (UTI) Working Group of the Health Care Office (HCO) of the European Association of Urology (EAU). Eur Urol 40:576–588
Article
PubMed
CAS
Google Scholar
Vicari E, La Vignera S, Calogero AE (2002) Antioxidant treatment with carnitines is effective in infertile patients with prostatovesiculoepididymitis and elevated seminal leukocyte concentrations after treatment with non-steroidal ant-inflammatory compounds. Fertil Steril 78:1203–1208
Article
PubMed
Google Scholar
Skau PA, Folstad I (2003) Do bacterial infections cause reduced ejaculate quality? A meta-analysis of antibiotic treatment of male infertility. Behav Ecol 14:40–47
Article
Google Scholar
Henkel R, Schill WB (1998) Sperm separation in patients with urogenital infections. Andrologia 30(Suppl 1):91–97
PubMed
Google Scholar
De Geyter C, De Geyter M, Behre HM, et al (1994) Peroxidasepositive round cells and microorganisms in human semen together with antibiotic treatment adversely influence the outcome of in vitro fertilization and embryo transfer. Int J Androl 17:127–134
Article
PubMed
Google Scholar
Liversedge NH, Jenkins JM, Keay SD, et al (1996) Antibiotic treatment-based on seminal cultures from asymptomatic male partners in in vitro fertilization is unnecessary and may be detrimental. Hum Reprod 11:1227–1231
Article
PubMed
CAS
Google Scholar
Kastrop PM, de Graaf-Miltenburg LA, Gutknecht DR, Weima SM (2007) Microbial contamination of embryo cultures in an ART laboratory: sources and management. Hum Reprod 22:2243–2248
Article
PubMed
Google Scholar
Grunewald S, Baumann T, Paasch U, Glander HJ (2006) Capacitation and acrosome reaction in non-apoptotic spermatozoa. Ann NY Acad Sci 1090:138–146
Article
PubMed
Google Scholar
Cohen DR, Basu S, Randall JM, et al (2004) Sperm motility in men with spinal cord injuries is enhanced by inactivating cytokines in the seminal plasma. J Androl 25:922–925
Article
PubMed
Google Scholar
Fabiani R, Johansson L, Lundkvist O, Ronquist G (1994) Enhanced recruitment of motile spermatozoa by prostasome inclusion in swim-up medium. Hum Reprod 9:1485–1489
PubMed
CAS
Google Scholar
Chi HJ, Kim JH, Ryu CS, et al (2008) Protective effect of antioxidant supplementation in sperm preparation medium against oxidative stress in human spermatozoa. Hum Reprod 23:1023–1028
Article
PubMed
CAS
Google Scholar
Chan PJ, Jacobson JD, Corselli JU, Patton WC (2006) A simple zeta method for sperm selection-based on membrane charge. Fertil Steril 85:481–486
Article
PubMed
CAS
Google Scholar