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Rôle du peptide vasoactif intestinal (VIP) dans la stéroïdogenèse et le vieillissement testiculaire
Role of vasoactive intestinal peptide (VIP) in steroidogenesis and aging in mouse testis
Andrologie volume 17, pages 123–128 (2007)
Resume
Des travauxin vitro suggèrent que le VIP pourrait jouer un rôle important au niveau de la biosynthèse de testostérone. Nous avons démontré que des Souris chez lesquelles le gène codant pour le VIP est supprimé montrent des niveaux de testostérone sériques très réduits comparés aux animaux sauvages. Egalement, ces animaux transgéniques présentent une réduction des niveaux de FSH (Follicle-stimulating hormone) à l’âge de quatre mois.
L’étude de la structure testiculaire a révélé une légère augmentation du pourcentage de tubules dégénérés chez les animaux VIP-/-comparés aux animaux sauvages. Les animaux chez lesquels le gène codant pour le VIP a été supprimé, à l’âge de quinze mois, ont des niveaux de stéroïdogenèse bas comparés aux souris sauvages. De manière intéressante, la dégénérescence testiculaire est moins sévère chez les animaux VIP-/-comparée à celles des animaux sauvages.
L’ensemble de ces résultats suggère que: 1) le VIP est un facteur important pour la régulation de la biosynthèse de testostérone et la sécrétion de FSH et 2) VIP régule le vieillissement testiculaire.
Abstract
VIP (vasoactive intestinal peptide) neuropeptide has long been considered to be putative regulator of testicular functions.In vitro evidence suggests that VIP could play an important role in testosterone biosynthesis. However, the endogenous role of VIP on testicular functions remained to be demonstrated.
In C57BL/6 mice exhibiting complete disruption of the VIP gene, the authors observed that male fertility remained intact but serum testosterone levels were lower than those of WT littermates. At the age of 4 months, this phenotype was accompanied by reduced steroidogenesis due to inhibition of the expression of StAR (steroidogenic acute regulatory protein) and 3ßHSD (3ß-hydroxysteroid dehydrogenase) in the testis. In addition, serum levels of FSH (Follicle-stimulating hormone) but not LH (Luteinizing hormone) were reduced in young KO males. Testicular anatomy also revealed a subtle but significantly higher percentage of degenerated seminiferous tubules in 4-month-old VIP-/-animals compared to WT.
In aging animals (15 months old), control males showed typical testicular aging including severe degeneration of seminiferous tubules, a dramatic decrease in serum testosterone levels and a reduction in StAR and 3ß-HSD gene expression. In age-matched VIP-/-males, serum levels of testosterone and steroidogenic enzymes were still very low. Interestingly, in contrast with young mice, testicular degeneration at 15 months was significantly less severe marked in VIP-/-mice than in WT mice. Altogether, these results suggest that: 1) VIP is an important factor for regulating testosterone biosynthesis and FSH secretion and 2) VIP regulates testicular aging.
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Lacombe, A., Lelievre, V., Roselli, C.E. et al. Rôle du peptide vasoactif intestinal (VIP) dans la stéroïdogenèse et le vieillissement testiculaire. Androl. 17, 123–128 (2007). https://doi.org/10.1007/BF03041165
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DOI: https://doi.org/10.1007/BF03041165
Mots clés
- neuropeptide
- biosynthèse de testostérone
- vieillissement testiculaire
- Luteinizing Hormone
- Follicle Stimulating Hormone