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Incidences de la restriction calorique et du Nickel sur l’aromatase testiculaire du rat

Impact of caloric and nickel restriction on testicular aromatase in the rat

Andrologie volume 17, pages 321–328 (2007)Cite this article

Resume

Des études épidémiologiques chez l’homme ont montré qu’il existait, depuis 1960, des signes d’augmentation de certaines pathologies génitales masculines. La contamination croissante de l’environnement par des composés chimiques semble être un facteur de causalité. Par ailleurs, divers auteurs avancent l’hypothèse que la restriction calorique a un effet bénéfique sur la reproduction masculine.

Ce travail a pour objectif de comparer les effets du nickel sur les processus de reproduction, entre autres l’étude de l’aromatase, chez des rats mâles nourris soit tous les jours, soit un jour sur deux, afin d’évaluer les possibles effets bénéfiques, ou non, d’une restriction calorique sur la fertilité des rats mâles.

Dans ce but, nous avons utilisé des rats mâles de souche « Wistar » nourris, soit tous les jours (groupe N), soit un jour sur deux (jeûne intermittent) (groupe J). Après un mois de ce traitement, les rats des deux lots (N) et (J) sont répartis chacun en deux groupes: l’un injecté intra-péritonéalement avec du NaCI 9‰ (groupes NO et JO), l’autre injecté intra-péritonéalement avec du NiCl2 à raison de 4mg/kg (groupes NNi et JNi). Le jeûne intermittent se poursuit parallèlement au traitement par le nickel, et ceci durant 1, 3, 5 et 10 jours.

Nous avons choisi pour notre étude comme biomarqueurs sexuels: dosage sérique des oestrogènes, dosage des ARN totaux et l’exploration moléculaire de l’ARNm de l’aromatase testiculaire.

L’étude de l’aromatase montre que le nickel seul stimule l’activité de l’aromatase testiculaire et provoque la dégradation des ARN totaux durant le 3èmeet le 10ème jours de traitement.

Le jeûne intermittent seul n’induit pas de variations significatives. Dans le cas où le nickel est associé au jeûne intermittent, il semble que pour le taux des ARN totaux testiculaires, l’effet du jeûne l’emporte, pas de dégradation des ARN totaux, d’où ses effets bénéfiques en protégeant les ARN totaux des effets délétères du nickel. On note aussi une surexpression de l’ARNm de l’aromatase vers le 10ème jours de traitement.

L’effet hypocalorique du jeûne intermittent pourrait être à l’origine de l’inhibition des effets cytotoxiques du nickel, métal classé parmi les stress oxydants.

Abstract

This study was designed to determine whether intermittent fasting induces malnutrition that, according to many authors, accentuates the cytotoxic effects of environmental pollutants, or caloric restriction that reduces these effects.

Ninety six male Wistar rats (180g) were divided into two groups: one group was fed daily (N) and the other group was fed every second day (J) for one month. At the end of one month, each group was then divided into two subgroups, one subgroup received an injection of 0.9% NaCI (groups NO and JO), the other subgroup received an injection of 4 mg/kg NiCIb2 (groups NNi and JNi).

Intermittent fasting was continued in parallel to treatment for 1, 3, 5 and 10 days.

Under these experimental conditions, nickel increased testicular aromatase activity and altered total RNA, while no alteration of these biomarkers was observed with intermittent fasting.

The combination of these two factors, nickel and intermittent fasting, did not amplify these effects. In contrast, protection of RNA by intermittent fasting was observed, especially overexpression of aromatase mRNA.

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Authors and Affiliations

  1. Laboratoire d’écophysiologie Animale, Faculté des Sciences de Sfax, Tunisie

    Najla Hfaiedh, Mohamed Salah Allagui & Abdelfettah el Feki

  2. Unité de recherche biochimie macromoléculaire et génétique, Faculté des Sciences de Gafsa, Tunisie

    Najla Hfaiedh & Abdelfettah el Feki

  3. Laboratoire de Biochimie IBFA, Biochimie- Université, US C2006 INRA, Esplanade de la Paix, EA 2608, 14032, Caen, France

    Serge Carreau

  4. Laboratoire de Biologie Cellulaire & Pollution, Faculté de Médecine de Toulouse, Université Paul Sabatier, 37 allées Jules Guesde, 31073, Toulouse, France

    Françoise Croute

  5. Laboratoire de biophysique, Faculté de Médecine de Sfax, Service Médecine nucléaire, CHU Habib Bourguiba de Sfax, Tunisie

    Fadhel Geurmazi

Authors
  1. Najla Hfaiedh
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  2. Mohamed Salah Allagui
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  3. Françoise Croute
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  4. Fadhel Geurmazi
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  5. Abdelfettah el Feki
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  6. Serge Carreau
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Correspondence to Serge Carreau.

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Hfaiedh, N., Allagui, M.S., Croute, F. et al. Incidences de la restriction calorique et du Nickel sur l’aromatase testiculaire du rat. Androl. 17, 321–328 (2007). https://doi.org/10.1007/BF03040364

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Basic and Clinical Andrology

ISSN: 2051-4190