- Assistance Medicale à la Procréation
- Published:
Tests de Selection des Spermatozoïdes avant Assistance Médicale à la Procréation
Selection Procedures of spermatozoa before assisted reproductive technologies
Andrologie volume 9, pages 286–296 (1999)
Resume
Les techniques de sélection des spermatozoïdes humains sont nombreuses et souvent spécifiques du centre d’andrologie qui les pratique. Nous ne faisons pas ici l’inventaire de toutes ces techniques mais reprenons les principales, le lavage-centrifugation, les méthodes de filtration («SpermPrep»), la migration ascendante ainsi que les gradients de densité avec des solutions colloïdales («Percoll», «PureSperm» et «Isolate») ou avec de nouvelles molécules iodées non colloïdales («OptiPrep»). Nous tentons de faire une synthèse de la littérature afin de dégager des méthodologies standardisées par technique et nous discutons les avantages et les inconvénients de chacune d’elle. La France étant un pays où les milieux utilisés pour une assistance médicale à la procréation doivent avoir été agréés d’un point de vue microbiologique, certains milieux décrits ici («Percoll») n’ont pas reçu cet agrément et ne doivent pas être utilisés en thérapeutique alors qu’un usage diagnostique est possible.
Certaines anomalies du sperme nécessitent un type de préparation particulière. Nous décrivons les techniques les plus consensuelles pour traiter les spermes avec anticorps anti-spermatozoïdes, avec présence de leucocytes ou suspects d’infection, les spermes hypervisqueux, ou encore le recueil des spermatozoïdes dans l’urine quand il existe une éjaculation rétrograde. Nous décrivons plus en détail une technique de préparation des spermes cryoconservés en s’inspirant des résultats de recherches fondamentales sur la congélation-décongélation des gamètes mâles humains.
Enfin, nous proposons une conduite à tenir pour le choix d’une technique d’AMP à proposer à un couple en tenant compte des caractéristiques du sperme, du résultat du test de sélection spermatique ainsi que de la survie des gamètes.
Abstract
There are many procedures to select spermatozoa but those procedures are often specific of each andrology centre. This review that is not an exhaustive review of the numerous methods published is rather devoided to focus on the main techniques, the sperm washing, the filtration methods («SpermPrep»), the swim-up, and the discontinuous density gradients with colloïdal solutions («Percoll», PureSperm” and «Isolate») including iodinated organic molecule as ‘OptiPrep». The first part presents a synthesis of literature in order to bring out standardized procedures, and a summary of the advantages and disadvantages of each step of these procedures.
Some specific anomalies of sperm need particular treatments. There is a consensus on the validated techniques to prepare semen samples in the presence of antisperm antibodies, leukocytes, bacterial contaminants, and also semen samples exhibiting hyperviscosity or in case of retrograde ejaculation. Cryopreserved sperm preparation is also described with references to experimental results in freezing-thawing human spermatozoa. The second part proposes guidelines for the therapeutic choice of the appropriate assisted reproductive technologies (i.e.: intra uterine artificial insemination, in vitro fertilization or intracytoplasmic sperm injection) according to semen parameters, and to the outcome of sperm selection and survival.
References
AITKEN R.J., CLARKSON J.S.: Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J. Reprod. Fertil. 1987, 81: 459–469.
AITKEN R.J., CLARKSON J.S.: Significance of reactive oxygen species and anti oxidants in defining the efficacy of sperm preparation techniques. J Androl., 1989, 9: 367–376.
AITKEN R.J., CLARKSON J.S., FISHEL S.: Generation of reactive oxygen species, lipid peroxidation and human sperm function. Biol. Reprod., 1989, 41: 183–197.
AITKEN R.J., WEST K.M.: Analysis of the relationship between reactive oxygen species production and leucocyte infiltration in fractions of human semen separated on Percoll gradients. Int. J. Androl., 1990, 13: 433–451.
AKERLOF E., FREDRICSSON B., GUSTAFSSON O., et al.: Comparison between a swim-up and a Percoll gradient technique for the separation of human spermatozoa. Int. J. Androl., 1987, 10: 663–669.
ALTURA R.M., CARELLA A., ALTURA B.T.: Adverse effects of TRIS.HEPES and MOPS buffers on contractile response of arterial and venous smooth muscle induced by prostaglandins. Prostaglandins Med., 1980, 5: 123–130.
ALVAREZ J.G., LASSO J.L., BLASCO L. et al.: Centrifugation of human spermatozoa induces sublethal damage; separation of human spermatozoa from seminal plasma by a dextran swim-up procedure without centrifugation extends their motile lifetime. Hum. Reprod., 1993, 8: 1087–1092.
KAKER H.W.G., BRINDLE J., IRVINE D.S., AITKEN R.J.: Protective effects of antioxidants on the impairment of sperm motility by activated polymorphonuclear leukocytes. Fertil. Steril., 1996, 65: 411–419.
BERGER T., MARRS R.P., MOYER D.L.: Comparison of techniques for selection of motile spermatozoa. Fertil. Steril., 1985, 43: 268–273.
BOLTON V.N., BRAUDE P.R.: Preparation of human spermatozoa for in vitro fertilization by isopycnic centrifugation on self generating density gradients. Arch. Androl., 1984, 13: 167–176.
BOLTON V.N., WARREN R.E., BRAUDE P.R.: Removal of bacterial contaminants from semen for in vitro fertilization or artificial insemination by the use of buoyant density centrifugation. Fertil. Steril. 1986, 46: 1128–1132.
BOWMAN C.M., BERGER E.M., BUTLER E.N., TOTH K.M., REPINE J.E.: HEPES may stimulate cultured endothelial cells to make growth-retarding oxygen metabolites. In Vitro Cell Dev. Biol. Steril. 1985, 21 Pt1: 140–142.
CALAMERA J.C., QUIROS M.C., BRUGO S., NICHOLSON R.F.: Comparison between swim-up and glass bead column techniques for the separation of human spermatozoa. Andrologia, 1991, 23: 259–261.
CENTOLA G.M., HERKO R., ANDOLINA E., WEISENSEL S.: Comparison of sperm speration methods: effects on recovery, motility, motion, parameters and hyperactivation. Fertil. Steril., 1998, 70: 1173–1175.
CHAN Y.N., ABUZEID M.I., MALCOMNSON J.H., SASY M.A.: Selection of human spermatozoa by a hyperosmotic two-layer Percoll gradient. Fertil. Steril., 1994, 61: 1097–1102.
CHAN S.Y.W., CHAN Y.M., TUCKER M.J.: Comparison of characteristics of human spermatozoa selected by the multiple tube swim-up and simple discontinuous Percoll gradient centrifugation. Andrologia, 1991, 23: 213–218.
CHAN S.Y.W., TUCKER M.J.: Differential sperm performance as judged by the zona free hamster egg penetration test relative to differing sperm penetration techniques. Hum. Reprod., 1992, 7: 255–260.
CHECK J.H., ZAVOS P.M., KATSOFF D., KIEFER D.: Effects of Percoll Discontinuous Density Gradients vs Sperm prep II vs Sephadex G-50 gel infiltration on semen parameters. Arch. Androl., 1993, 31: 69–73.
CLAASSENS O.E., MENKVELD R., HARRISO K.L.: Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation. Hum. Reprod., 1998, 13: 3139–3143.
COWAN A.I., MARTIN R.L.: Ionic basis of the membrane potential response of rat dorsal vagal motoneurones to HEPES buffer. Brain Res., 1996, 717: 69–75.
CRICH J.P., JEQUIER A.M.: Infertility in men with retrograde ejaculation: the action of urine on sperm motility and a simple method for achieving antegrade ejaculation. Fertil. Steril., 1978, 30: 572–576.
DANIEL P.F.: Incorporation of labeled glucosamine into glycoproteins by organ cultures of hamster trachea: adverse effects of HEPES buffer. In Vitro., 1975, 11: 347–353.
DAYA S., GWATKIN R.B.L.: Improvement in semen quality using glass bead column. Arch. Androl., 1987, 18: 241–244.
DAYA S., GWATKIN R.B.L., BISSESSAR H.: Separation of motile human spermatozoa by means of a glass bead column. Gam. Res., 1987, 17: 375–380.
DE VOS A., NAGY Z.P., VAN DE VELDE H., JORIS H., BOCKEN G., VAN STEIRTEGHEM A.: Percoll gradient centrifugation can be omitted in sperm preparation for intracytoplasmic sperm injection. Hum. Reprod., 1997, 12: 1980–1984.
DODSON W.C., MOESSNER J., MILLER J. et al.: A randomized comparison of the methods of sperm preparation for intra-utérine insemination. Fertil. Steril., 1998, 70: 574–575.
DRAVLAND J.E., MORTIMER D.: A simple discontinuous Percoll gradient for washing human spermatozoa. IRCS Med. Sci., 1985, 13: 16–17.
FORMAN R., GUILLET-ROSSO F., FARI A., et al. — Importance of semen preparation in avoidance of reduced in vitro fertilization results attributable to bacteria. Fertil. Steril., 1987, 47: 527–530.
FORSTER M.S., SMITH W.D., LEE W.I., et al.: Selection of human spermatozoa according to their relative motility and their interaction with zona free hamster eggs. Fertil. Steril., 1983, 40: 655–660.
GELLERT-MORTIMER S.T., CLARKE G.N., BAKER H.W.G., et al.: Evaluation of Nycodenz R and Percoll density gradients for the selection of motile human spermatozoa. Fertil. Steril., 1988, 49: 335–341.
GILMORE J.A., LIU J., GAO D.Y., CRITSER J.K.: Determination of optimal cryoprotectants and procedures for thei addition and removal from human spermatozoa. Hum. Reprod., 1997, 12: 112–118.
GOMEZ E., AITKEN J.: Impact of in vitro fertilization culture media on peroxidative damage to spermatozoa. Fertil. Steril., 1996, 65: 880–882.
GONZALES J., MATHIEU C., ZERAH S., VERDAGUER S.: Sperme, germes et PMA: une enquête des BLEFCO. Reprod. Hum. Horm., 1992, 3: 256–259.
GORUS F.K., PIPELEERS D.G.: A rapid method for the fractionation of human spermatozoa according to their progressive motility. Fertil. Steril., 1981, 35: 662–665.
GRIVEAU J.F., LE LANNOU D.: Effects of antioxidants on human sperm preparation techniques. Int. J. Androl., 1994, 17: 225–231.
HATTORI H., YAMAGUCHI F, OHNO H. AND NOMURA M.: Antigenecity study of iodixanol, a new non-ionioc contrast medium. J. of Toxicological Science 1995; 20: suppl. 1, 117–123.
HENSLEIGH H.C., JAVKIN P.D., TAGATZ G.E., PRYOR J.L.: Evaluation for antisperm antibodies after storage of sperm in TEST-YOLK Buffer. Fertil. Steril., 1996, 66: 454–458.
HUGHES C.M., LEWIS S.E.M., McKELVEY, MARTIN V.J., THOMPSON W.: The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum. Reprod., 1998, 13: 1240–1247.
JEULIN C., FENEUX D., SERRES C., et al.: Sperm factors related to failure of human in vitro fertilization. J. Reprod. Fertil., 1986, 76: 735–744.
JONES R.C., HOLT W.V.: The effects of washing on the ultrastructure and cytochemistry of ram spermatozoa. J. Reprod. Fertil., 1974, 41: 159–167.
JOUANNET P., SOUMAH A.: Ejaculation retrograde et fertilité in l’éjaculation et ses perturbations. J. Buvat, P. Jouannet Eds SIMEP—Lyon., 1984, 62–68,
KANWAR K.C., YANAGIMACHI R., LOPATA A.: Effects of human seminal plasma on fertilizing capacity of human spermatozoa. Fertil. Steril., 1979, 31: 321–327.
KIRSCH M., LOMONOSOVA E.E., KORTH H.G., SUSTMANN R., DE GROOT H.: Hydrogen peroxide formation by reaction of peroxynitrite with HEPES and related tertiary amines. Implications for a general mechanism. J. Biol. Chem., 1998, 273: 12716–12724.
LEE W.I., GADDUM-ROSSE P., SMITH W.D., STEACHEVER M., BLANDAU R.J.: Laser light scattering study of the effect of washing on sperm motility. Fertil. Steril., 1982, 38: 62–67.
LE LANNOU D., BLANCHARD Y.: Nuclear maturity and morphology of human spermatozoa selected by Percoll density gradient centrifugation or swim-up procedure. Reprod. Fertil., 1988, 84: 551–556.
LESSLEY B.A., GARNER D.L.: Isolation of motile spermatozoa by density gradient centrifugation in Percoll. Gam. Res., 1983, 7: 49–61.
LEU P.L., REBEL G.: Effect of HEPES on the taurine uptake by cultured glial cells. J. Neurosci. Res., 1989, 23: 78–86.
MAHADEVAN M., BAKER G.: Assessment and preparation of semen for in vitro fertilization in Wood C., Trounson A. Eds. Clinical In vitro Fertilization. Springer Verlag. Berlin, 1984, p. 83–97.
MAHADEVAN M.M., BATRES F., MILLER M.M., MOUTOS D.M.: Yeast infection of sperm, oocytes and embryos after intra-vaginal culture for embryo transfer. Fertil. Steril., 1996, 66: 481–483.
MAKLER A., DAVID R., BLUMENFELD Z., BETTER O.S.: Factors affecting sperm motility. VII. Sperm viability as affected by change of pH and osmolarity of semen and urine specimens. Fertil. Steril., 1981, 36: 507–511.
MAKLER A., JACOBI P.: Effects of shaking and centrifugation on human sperm motility. Arch. Androl., 1981, 7: 2–6.
MAKLER A., STOLLER J., MAKLER-SHIRAN E.: Dynamics aspects concerned with the mecanism of separating motile sperm from non motile sperm, leucocytes and debris with the use high density Percoll gradients. Fertil. Steril., 1998, 70: 961–966.
McCLURE R.D., NUNES L., TOM R.: Semen manipulation: improved sperm recovery and function with a two layer Percoll gradient. Fertil. Steril., 1989, 51: 874–877.
MORTIMER D.: Sperm recovery techniques to maximize fertilizing capacity. Reprod. Fertil. Dev., 1994, 6: 25–31.
MORTIMER D.: Sperm preparation techniques and iatrogenic failures of in vitro fertilization. Hum. Reprod., 1991, 6: 173–176.
O.M.S.: Manuel de laboratoire de l’OMS. Analyse du sperme humain et de l’interaction des spermatozoïdes avec le mucus cervical. Editions INSERM Paris, 1993.
ORD T., PATRIZIO P., MARELLO E., BALMACEDA J.P., ASCH R.H.: Mini-Percoll: a new method of semen preparation for IVF in severe male factor infertility. Hum. Reprod., 1990, 5: 987–989.
OSHIO S.: Apparent densities of spermatozoa of various mammalian species. Gametes Research, 1988, 20: 1257–1259.
PARINAUD J., LE LANNOU D., VIEITEZ G., GRIVEAU J.F., MILHET P., RICHOILLEY G.: Enhancement of motility by treating spermatozoa with an antioxidant solution («Sperm-Fit») following ejaculation. Hum. Reprod., 1997, 12: 2434–2436.
PAULSON J.D., POLAKOSKI K.L.: A glass wool column procedure for removing extraneous material from the human ejaculate. Fertil. Steril., 1977, 28: 178–181.
PERTOFT H., LAURENT T.C., LAAS T.: Density Gradients prepared from colloïdal silica particles coated by polyvynyl pyrolidone (Percoll). Anal. Biochem., 1978, 88: 271–282.
PICKERING S.J., FLEMING T.P., BRAUDE P.R., BOLTON V.N., GRESHAM G.A.G.: Are human spermatozoa separated on a Percoll density gradient safe for therapeutic use? Fertil. Steril., 1989, 51: 1024–1029.
PUNJABI U., GERRIS J., VANBIJIEN J., DELBEKE L., GIELIS M., BUYTAERT P.H.: Comparison between different pre-treatment techniques for sperm recovery prior to intra uterine insemination, GIFT or IVF. Hum. Reprod., 1990, 5: 75–83.
POOLE C.A., REILLY H.C., FLINT M.H.: The adverse effects of HEPES, TRIS, TES, and BES zwitterion buffers on the ultrastructure of cultured embryo epiphyseal chondrocytes. In Vitro, 1982, 18: 755–765.
PRAKASH P., LEYKIN L., CHEN Z., et al.: Preparation by differential gradient centrifugation is better than swin-up in selecting sperm with normal morphology (strict criteria). Fertil. Steril., 1998, 69: 722–726.
RUSSEL L.D., ROGER S.T.: Improvement in the quality and fertilization potential of a human sperm population using the rise technique. Androl., 1987, 8: 25–33.
SCOTT L., SMITH S.: Mouse in vitro fertilization, embryo development and viability, and human sperm motility in substances used for human sperm preparation for assisted reproduction. Fertil. Steril., 1997, 67: 372–381.
SHERMAN J.K., PAULSON J.D., LIU K.C.: Effect of glass wool filtration on ultrastructure of human spermatozoa. Fertil. Steril., 1981, 36: 643–647.
SIMPSON J.A., CHEESEMAN K.H., SMITH S.E., DEAN R.T.: Free-radical generation by copper ions and hydrogen peroxide. Stimulation by HEPES buffer. Biochem. J., 1988, 254: 519–523.
SOFIKITIS N., MIYAGAWA I., ZAVOS P.M.: Selection of single stranded deoxyribo nucleic acid spermatozoa via Sperm Prep filtration column. Fertil. Steril. 1993, 59: 690–692.
STERZIK K., DE SANTO M., UHLICH S., GAGSTEIGER F., STREHLER E.: Glass wool filtration leads to a higher percentage of spermatozoa with intact acrosomes: an ultrastructural analysis. Hum. Reprod., 1998, 13: 2506–2511.
TANPHAICHITR N., MILLETTE C.F., AGULNICK A., FITZGERALD L.M.: Egg penetration ability and structural properties of human sperm prepared by Percoll gradient centrifugation. Gam. Res., 1988, 20: 67–81.
TEA N.T., JONDET M., SCHOLLER R.: Procédé d’isolement des spermatozoïdes mobiles du sperme humain par la méthode de migration-sédimentation. Pathol. Biol., 1983, 31: 688–690.
TOMLINSON M.J., BARRATT C.L.R., BOLTON A.E., et al.: Round cells and sperm fertilizing capacity: the presence of immature germ cells but not seminal leukocytes are associated with reduced sucess of in vitro fertilization. Fertili. Steril. 1992, 58: 1257–1259.
VAN DER BERGH M., REVELARD P., BERTRAND E., BIRAMANE J., VANIN A.S., ENGLERT Y.: Glass wool column filtration, an advantageous way of preparing semen samples for intracytoplasmic sperm injection: an auto-controlled randomized study. Hum. Reprod., 1997, 12: 509–513.
VAN DER VEN H.H., JEYENDRAN R.S., ALHASANI S., et al.: Glass wool column filtration of human semen: relation to swim-up procedure and outcome of IVF. Hum. Reprod., 1988, 3: 85–88.
VELEZ DE LA CALLE J.F. — Human spermatozoa selection in improved discontinuous Percoll gradients. Fertil. Steril., 1991, 56: 737–742.
WONG P.C., BALMACEDA J.P., BLANCO J.D., GIBBS R.S., ASCH R.H.: Sperm washing and swimup technique using antibiotics removes microbes from human semen. Fertil. Steril., 1986, 45: 97–100.
YAMAMOTO D., SUZUKI N.: Blockage of chloride channels by HEPES buffer. Proc. R. Soc. Lond. B. Biol. Sci., 1987, 230: 93–100.
ZAVOS P.M., CENTOLA G.M.: Améliorations séminales des spermatozoïdes utilisés pour la fécondation artificielle. Comparaison entre la méthode de nage ascendante et une nouvelle méthode de filtration du sperme sur colonne. Contracept. Fertil. Sex., 1990, 18: 943–948.
ZAVOS P.M., CORREA J.R., ZARMAKOUPISZAVOS P.M.: Antisperm antibody treatment mode: levels of antisperm antibodies after incubation with TEST-YOLK Buffer and filtration using the SpermPrep II” method. Fertil. Steril., 1998, 69: 517–521.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mathieu, C., Lucas, H. Tests de Selection des Spermatozoïdes avant Assistance Médicale à la Procréation. Androl. 9, 286–296 (1999). https://doi.org/10.1007/BF03035240
Issue Date:
DOI: https://doi.org/10.1007/BF03035240
Mots clés
- Sperme humain
- préparation du sperme
- test de migration survie
- gradients de densité
- Percoll
- migration ascendante