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Restauration de la fertilité masculine par la spermatogenèsein vitro

Restoration of male fertility byin vitro maturation of germ cells

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Résumé

Une technique de culturein vitro, préalablement testée sur les cellules germinales provenant d’hommes avec spermatogénèse complète, a été appliquée, à des fins thérapeutiques, aux cas d’arrêt de maturation méiotique et post méiotique. Un petit nombre de spermatocytes primaires provenant d’hommes avec arrêt au stade de pachytène de la première division méiotique, s’est développé jusqu’aux spermatides; ces spermatides ont été capables de féconder les ovocytes et d’être à l’origine d’embryons qui ont évolué ensuite jusqu’au terme. Dans d’autres cas, des spermatides bloquées avant le processus de spermiogénèse ont pu se développer jusqu’aux spermatides allongées fécondes, et les premières naissances dans des cas d’arrêt complet de spermiogénèse ont pu ainsi être obtenues.

Abstract

Anin-vitro culture technique, previously tested with germ cells from men with complete spermatogenesis, was applied in assisted reproduction treatment for cases of meiotic and postmeiotic maturation arrest. Some primary spermatocytes from men with maturation arrest at the pachytene stage developed up to the late elongated spermatid stages and were capable of fertilizing the spouse’s oocytes and of giving rise to embryos that were transferred into the spouse’s uterus and subsequently developed to term. In other cases, round spermatids blocked in vivo before the process of spermiogenesis developed to elongated spermatidsin vitro; with the use of suchin-vitro formed spermatids, the first term pregnancies in cases of complete spermiogenesis arrest were achieved. These findings show that certain in-vivo developmental blocks in male germ cells from patients with severe testiculopathies can be overcome byin-vitro culture, probably by modifying control mechanisms acting at developmental checkpoints.

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Correspondence to J. Tesarik.

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Tesarik, J. Restauration de la fertilité masculine par la spermatogenèsein vitro . Androl. 10, 289–291 (2000) doi:10.1007/BF03034751

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Mots clés

  • Spermatogénèse in vitro
  • azoospermie sécrétoire
  • arrêt de maturation
  • spermatocyte
  • spermatide
  • procréation médicalement assistée

Key words

  • In vitro spermatogenesis
  • non obstructive azoospermia
  • maturation arrest
  • spermatocyte
  • spermatid
  • assisted reproductive technology