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Le transfert intratesticulaire de cellules germinales immatures

Intratesticular transplantation of immature germ cells

Andrologie volume 12, pages 347–350 (2002)Cite this article

Resume

La transplantation de cellules germinales mâles immatures (spermatogonies-souches) dans le testicule adulte permet d’obtenir une spermatogenèse complète lorsque les sujets donneurs et receveurs sont d’espèce identique ou très proche. Lorsque la distance phylogénétique est trop grande, les cellules germinales survivent, en liaison avec les cellules de Sertoli, mais ne s’engagent pas dans la méiose. Cette méthode, développée chez les rongeurs, permet également d’obtenir des gamètes transgéniques. Son application chez l’homme devrait permettre de rendre la fertilité à des patients traités pendant l’enfance par des traitements anticancéreux et pour lesquels des cellules prélevées par biopsie testiculaire ont été cryoconservées.

Abstract

Immature male germ cells (spermatogonial stem cells) are able to develop and differentiate in the adult testis of the same or closely related species. Spermatozoa of several species of rodents can be obtained from stem cells transplanted into the testes of mice. When the two species are not too phylogenetically different, germ cells can proliferate, but they do not enter into meiosis.

Germ cell transfer, first performed in rodents by Bringer, is a very useful method to study cell relations during spermatogenesis and provides a good method for production of transgenic gametes.

In human therapy, it should be possible to restore the fertility of young adult men treated for cancer during childhood, as chemotherapy and radiotherapy induce the death of germ cells. If cryoperservation of testicular tissue is performed before starting gonadotoxic therapy, stem cells can subsequently repopulate the seminiferous tubules.

References

  1. ASLAM I., FISHEL S., MOORE H., DOWELL K., THORNTON S.: Fertility preservation of boys undergoing anti-cancer therapy: a review of the existing situation and prospects for the future. Hum. Reprod., 2000, 15: 2154–2159.

    Article  PubMed  CAS  Google Scholar 

  2. BAHADUR G., RALPH D.: Gonadal tissue cryopreservation in boys with paediatric cancers. Hum. Reprod., 2000, 14: 11–17.

    Article  Google Scholar 

  3. BRINSTER R.L., AVARBOCK M.R.: Germline transmission of donor haplotype following spermatogonial transplantation. Proc. Nat. Acad. Sci., 1994, 91: 11303–11307.

    Article  PubMed  CAS  Google Scholar 

  4. BRINSTER R.L., ZIMMERMANN J.W.: Spermatogenesis following male germ-cell transplantation. Proc. Nat. Acad. Sci., 1994, 91: 11298–11302.

    Article  PubMed  CAS  Google Scholar 

  5. BRINSTER R.L.: Germline stem cell transplantation and transgenesis. Science, 2002, 296: 2174–2176.

    Article  PubMed  CAS  Google Scholar 

  6. CLOUTHIER D.E., AVARBOCK M.R., MAIKA S.D., HAMMER R.E., BRINSTER R.L.: Rat spermatogenesis in mouse testis. Nature, 1996, 381: 418–421.

    Article  PubMed  CAS  Google Scholar 

  7. DE ROOIJ D.G.: Stem cells in the testis. Intern. J. Exp. Pathol., 1998, 79: 67–80.

    Article  Google Scholar 

  8. DYM M.: Spermatogonial stem cells of the testis. Proc. Nat. Acad. Sci., 1994, 91: 11287–11289.

    Article  PubMed  CAS  Google Scholar 

  9. HONARAMOOZ A., SNEDAKER A., BOIANI M., SCHOLER H., DOBRINSKI I., SCHLATT S.: Sperm from neonatal mammalian testes grafted in mice. Nature, 2002, 418: 778–781.

    Article  PubMed  CAS  Google Scholar 

  10. HOVATTA O.: Cryopreservation of testicular tissue in young cancer patients. Hum. Reprod. Update, 2001, 7: 378–383.

    Article  PubMed  CAS  Google Scholar 

  11. IZADYAR F., CREEMERS L.B., VAN DISSEL-EMILIANI F.M., VAN PELT A.M., DE ROOIJ D.G.: Spermatogonial stem cell transplantation. Mol. Cell. Endocr., 2000, 169: 21–26.

    Article  CAS  Google Scholar 

  12. JIANG F.X.: Male germ cell transplantation: promise and problems. Reprod. Fertil. Dev., 2001, 13: 609–614.

    Article  PubMed  CAS  Google Scholar 

  13. JOHNSTON D.S., RUSSELL L.D., GRISWOLD M.D.: Advances in spermatogonial stem cell transplantation. Rev. Reprod., 2000, 5: 183–188.

    Article  PubMed  CAS  Google Scholar 

  14. NAGANO M., MC CARREY J.R., BRINSTER R.L.: Primate spermatogonial stem cell colonize mouse testes. Biol. Reprod., 2001, 64: 1409–1416.

    Article  PubMed  CAS  Google Scholar 

  15. OATLEY J.M., DE AVILA D.M., MCLEAN D.J., GRISWOLD M.D., REEVES J.J.: Transplantation of bovine germinal cells into mouse testes. J. Anim. Sci., 2002, 80: 1925–1931.

    PubMed  CAS  Google Scholar 

  16. OGAWA T.: Spermatogonial transplantation technique in spermatogenesis research. Intern. J. Androl., 2000, 23, suppl 2: 57–59.

    Article  Google Scholar 

  17. OGAWA T.: Spermatogonial transplantation: the principle and possible applications. J. mol. Med., 2001, 79: 368–374.

    Article  PubMed  CAS  Google Scholar 

  18. OGAWA T., DOBRINSKI I., AVARBOCK M.R., BRINSTER R.L.: Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes. Biol. Reprod., 1999, 60: 515–521.

    Article  PubMed  CAS  Google Scholar 

  19. REIS M.M., TSAI M.C., SCHLEGEL P.N.et al.: Xenogeneic transplantation of human spermatogonia. Zygote, 2000, 8: 97–105.

    Article  PubMed  CAS  Google Scholar 

  20. SCHLATT S., FOPPIANI L., ROLF C., WEINBAUER G.F., NIESCHLAG E.: Germ cell transplantation into X-irradiated monkey testes. Hum. Reprod., 2002, 17: 55–62.

    Article  PubMed  CAS  Google Scholar 

  21. SCHLATT S., ROSIEPEN G., WEINBAUER G.F., ROLF C., BROOK P.F., NIESCHLAG E.: Germ cell transfer into rat, bovine, monkey and human testes. Hum. Reprod, 1999, 14: 144–150.

    Article  PubMed  CAS  Google Scholar 

  22. SCHLATT S., VON SCHONFELDT V., NIESCHLAG E.: Germ cell transplantation in the male: animal studies with a human perspective. Hum. Fertil, 1999, 2: 143–148.

    Article  Google Scholar 

  23. SOUSA M., CREMADES N., ALVES C., SILVA J., BARROS A.: Developmental potential of human spermatogenis cells cocultured with Sertoli cells. Hum. Reprod., 2002, 17: 161–172.

    Article  PubMed  Google Scholar 

  24. THOMSON A.B., CRITCHLEY H.O., KELNAR C.J., WALLACE W.H.: Late reproductive sequelae following treatment of childhood cancer and options for fertility preservation. Best. Pract. Res. Clin. Endocr. Metabol., 2002, 16: 311–334.

    Article  Google Scholar 

  25. ZAPZALKA D.M., REDMON J.B., PRYOR J.L.: A survey of oncologists regarding sperm cryopreservation and assisted reproductive techniques for male cancer patients. Cancer, 1999, 86: 1812–1817.

    Article  PubMed  CAS  Google Scholar 

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  1. CECOS, Hôpital Tenon, 4 Rue de la Chine, 75020, Paris, France

    Eric Vendrely

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Vendrely, E. Le transfert intratesticulaire de cellules germinales immatures. Androl. 12, 347–350 (2002). https://doi.org/10.1007/BF03034652

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Basic and Clinical Andrology

ISSN: 2051-4190