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Mécanismes régulateurs des α-glucosidases épididymaires chez le rat

Regulatory mechanisms of α-glucosidases in rat epididymis


Epithelial cells of mammalian epididymis produced acid and neutral α-glucosidases (α-G) which are either modulated by hormonal systemic influences or by local factors arising from the rete testis fluid. In this study, ligation of ductuli efferentes in the rat was characterized by a modest elevation of both α-G 15 days after surgery. In adrenalectomized rats, both α-G are lower than in intact animals. Because of the continuance of spermatophagia 2 weeks after ductuli ligation, the observed enzymatic variations cannot be ascribed to paracrine influences; however, the effects of adrenalectomy on α-G levels are obvious and may be attributed to a multifactorial endocrine control (androgens and corticoids).


Les cellules épithéliales de l’épididyme des mammifères contiennent des α-glucosidases (α-G) acide et neutre dont la modulation obéit à un contrôle hormonal systémique ou encore local par le biais du fluide du rete testis. Dans ce travail, la ligature des ductuli efférentes chez ie rat est caractérisée par une élévation modeste des deux α-G 15 jours après la chirurgie. Chez des animaux surrénalectomisés, les activités α-G s’abaissent par référence aux valeurs retrouvées chez l’animal intact. Etant donné la persistance de spermatophagie deux semaines après ligature des ductuli, les variations enzymatiques observées sont difficilement attribuables à des influences paracrines; par contre, les effets de l’absence de corticostérone sur les α-G sont tangibles et reflètent un contrôle stéroïdien multifactoriel (androgènes et corticoïdes).


  1. 1

    -Alexander NJ. Ultrastructural changes in rat epididymis after vasectomy. Z. Zellforsched. 1973, 136: 177–182.

    Article  CAS  Google Scholar 

  2. 2

    -Back DJ. The presence of metabolites of 3H testosterone in the lumen of cauda epididymis of the rat. Steroids, 1975, 25: 413–420.

    PubMed  Article  CAS  Google Scholar 

  3. 3

    -Chapdelaine P, Tremblay RR, Dubé JY. Origine of maltase and variations in infertile men. Arch. Androl., 1978, 1: 61–68.

    PubMed  Article  CAS  Google Scholar 

  4. 4

    -Cooper TG, Weidner W, Nieschlag AG The influence of inflammation on the human male genital tract on secretion of the seminal markers α-glucosidase, glycerophosphocholine, carnitine, fructose and citric acid. Int. J. Andrology., 1990, 13: 329–336.

    Article  CAS  Google Scholar 

  5. 5

    -Flickinger CJ, Gallien TN, Spell DR. The incidence of spermatic granulomas and their relation to testis weight after vasectomy and vasovasostomy in Lewis rats. J. Androl, 1986, 7: 285–291.

    PubMed  CAS  Google Scholar 

  6. 6

    -Grandmont AM, Chapdelaine P, Tremblay RR. Presence of α-glucosidases in the male reproductive system of the rat and hormonal influences. Can. J. Biochem., 1983, 61:764–769.

    CAS  Article  Google Scholar 

  7. 7

    -Hoffer AP, Hamilton DW, Fawcett DW. Phagocytosis of spermatozoa by the epithelial cells of the ductuli efferentes after epididymal obstruction in the rat. J. Reprod. Fertil., 1975, 44: 1–9.

    PubMed  CAS  Article  Google Scholar 

  8. 8

    -Koskimies AI, Kormano M. The proteins in fluids from the semineferous tubules and rete testis of the rat. J. Reprod. Fertil., 1973, 34: 433–444.

    PubMed  CAS  Google Scholar 

  9. 9

    -Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 1951, 193: 265–275.

    PubMed  CAS  Google Scholar 

  10. 10

    -Marchand L., Frenette G, Tremblay RR. Fate of α-1,4 glucosidase and cathepsin D in the rat epididymis after vasectomy. Enzyme, 1990, 43:192–196.

    PubMed  CAS  Google Scholar 

  11. 11

    -Robaire B., Hermo L. Efferent dacts, epididymis, and vas deferens: structure, functions, and their regulation. The Physiology of Reproduction, Knobil E et Neil J eds, Raven Press, 1988, Chap. 23: 999–1081.

  12. 12

    -Shabanowitz RB, Killian GJ. Two dimensional electrophoresis of proteins in principal cells, spermatozoa, and fluid associated with the rat epididymis. Biol. of Reprod., 1987, 36: 753–768.

    Article  CAS  Google Scholar 

  13. 13

    -Tremblay RR. Epididymal markers and sperm analysis. In Andrology, Male Fertility and Sterility. Eds JD Paulson, A Negro-Vilar. E Lucena et L Martini, 1986: 273–292.

  14. 14

    -Tremblay RR, Demers P, Besançon J, Lemay JP. Alpha-1,4-glucosidase activity in ram seminal plasma is inversely related to serum testosterone. Enzyme, 1990, 43 (2): 107–111.

    PubMed  CAS  Google Scholar 

  15. 15

    -Vanha-Perttula T, Arya M. Lectin staining of rat testis and epididymis after ligation of excurrent ducts at different levels. Biol. Reprod., 1985, 33: 477–485.

    PubMed  Article  CAS  Google Scholar 

  16. 16

    -Vreeburg JTM. Distribution of testosterone and 5α-dihydrotestosterone in rat epididymis and their concentrations in efferent duct fluid. J. Endocrinol., 1975, 67: 203–210.

    PubMed  Article  CAS  Google Scholar 

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Correspondence to Julie Morisset.

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Morisset, J., Frenette, G. & Tremblay, R.R. Mécanismes régulateurs des α-glucosidases épididymaires chez le rat. Androl. 1, 53–55 (1991).

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Key words

  • α-glucosidase
  • epididymis
  • rat
  • hormones

Mots clefs

  • α-glucosidase
  • epididyme
  • rat
  • hormones