Effect of androgens on Sertoli cell maturation in human testis from birth to puberty

Table 2 Results of histological data for the 7 ages control, Complete Androgen Insentivity Syndrom (CAIS) and Leydig Cell Tumor (LCT) groups

Groups	Groups 1–7	Group 1	Group 2	Group 3	Group 4	Group 5	Group 6	Group 7	CAIS	LCT
Age range	0–16 y.	0–30 d.	1–3 m.	3–6 m.	6 m. - 3 y.	3–6 y.	6–12 y.	12–16 y.	3 m.- 20y.	6-10y.
Mean tubular diameter (μm)
n	84	12	12	12	12	12	11	13	4	4
median	75.0	75.0	75.0	80.0	75.0	65.0	65.0	170.0	57.5	137.5
[min-max]	[50–275]	[60–80]	[70–90]	[70–90]	[60–90]	[55–75]	[50–140]	[75–275]	[55–70]	[105–175]
Kruskal-Wallis/Mann-Whitney	p < 0.001	b	bc	c	bc	a	ab	d	a	d
S/T score
n	79	12	12	12	9	12	10	12	4	4
median	1.96	2.77	1.42	1,41	1.46	1,27	2.16	12.07	**	2.60
[min-max]	[0.4–28.2]	[0.88–6.42]	[0.68–4.58]	[0.4–3.06]	[0.88–2.2]	[0.86–3.88]	[0.66–5.08]	[2.84–28.2]	[0–2.1]**	[2,2–3.34]
Kruskal-Wallis/Mann-Whitney	p < 0.001	ab	ab	ab	a	ab	ab	c	**	b
Testicular Fertility Index
n	79	12	12	12	9	12	10	12	4	4
median	84.0	85.0	82.0	74.0	76.0	72.0	85.5	100.0	**	100.0
[min-max]	[34–100]	[50–99]	[40–100]	[34–90]	[58–94]	[42–100]	[42–100]	[88–100]	[0–95]**	[98–100]
Kruskal-Wallis/Mann-Whitney	p < 0.001	a	a	a	a	a	a	b	**	b
Most advanced germ cell
Spermatogonia (n)	73	12	12	12	12	12	10	3	1**	0
Spermatocyte (n)	2	0	0	0	0	0	0	2	0	0
Round spermatid (n)	2	0	0	0	0	0	1	1	0	2
Elongated spermatid (n)	4	0	0	0	0	0	0	4	0	1
Spermatozoa (n)	3	0	0	0	0	0	0	3	0	1
Fisher’s exact test	p < 0.001
Lumen
Absent (n)	71	12	12	12	12	12	9	2	4	0
Present in only some tubes (n)	4	0	0	0	0	0	2	2	0	3
Present (n)	9	0	0	0	0	0	0	9	0	1
Fisher’s exact test	p < 0.001
No Leydig cells
rare (n)	35	2	0	1	10	12	9	1	0	4 *
numerous (n)	49	10	12	11	2	0	2	12	4	0 *
Fisher’s exact test	p < 0.001

The Table gives the histological characteristics of the samples in each group for the following parameters:
- median tubular diameter (cord/tubular diameter)
-S/T score: number of spermatogonia per seminiferous cord/tubule transverse section,
- TFI: Tubular Fertility Index (percentage of the seminiferous cord/tubule cross-section containing at least onegerm cell),
- most advanced germ cell :it were significantly different in group 7 (12-16 y.o.) compared to the other age groups. CAIS samples exhibited a prepubertal pattern with mean tubular diameter lower than in 0-12 y.o. control boys and no lumen within the seminiferous cords.
Groups labeled with the same letters were not significatively different on pairwise comparison. P<0.05 wasconsidered as significant. (Kruskal-Wallis/Mann-Whitney)
n= number in the sample
min-max = minimum-maximum

ISSN: 2051-4190