Fig. 2

Effects of hypotaurine supplementation on sperm fertilization capacity after thawing. Numbers are averaged ± SEM for (A) P110 and P80 (arbitrary units: a.u.) and percent ± SEM for (B) acrosome integrity (%). “H -”: sperm cells were processed by density gradient centrifugation (DGC), washed and frozen without hypotaurine supplementation. “H +”: sperm cells were processed by DGC, washed and frozen in the presence of 50 mM of hypotaurine. After thawing, measurements were performed on (A) the levels of cryo-capacitation (n = 15), and (B) the percentage of altered or reacted (AR) spermatozoa (n = 25). Cryo-capacitation levels were evaluated by measuring the tyrosine phosphorylation of proteins P110 and P80 after thawing under capacitation conditions (37 °C and 5% CO₂). Conditions H+ and H- were compared using parametric or non-parametric tests based on statistical distribution and number of subjects (n).** indicates significant difference with p < 0.01 in comparison to the H- condition