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Fig. 4 | Basic and Clinical Andrology

Fig. 4

From: Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation

Fig. 4

Distribution of events detected in the quadrants of the flow cytometry charts by TUNEL (A) and 8-OHdG (B) evaluations in normal and abnormal semen samples. Quadrants Q1 to Q4 are as defined in Fig. 1. The data are presented as mean ± standard deviation. The addition of Q1 + Q2 + Q3 + Q4 represents 100% of the events detected. Note that in each case the events recorded in Q3 are always significantly greater in abnormal samples. In addition, as expected, there are significantly fewer negative TUNEL cells in the Q1 quadrant in the abnormal samples. As previously indicated by Vorilhon et al. [23] with regard to evaluation of 8-OHdG, the percentage of 8-OHdG-positive cells alone is insufficient to distinguish normal from abnormal samples (no significant differences in the Q1 and Q2 quadrants). The mean fluorescence intensity of the sample should also be taken into consideration [25]

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Basic and Clinical Andrology

ISSN: 2051-4190