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Fig. 2 | Basic and Clinical Andrology

Fig. 2

From: Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation

Fig. 2

A) Comparison of sperm DNA fragmentation between individuals with normal and abnormal sperm parameters. For TUNEL analysis, PI-negative were excluded by excluding Q3 and Q4 quadrant of the flow chart. Data are presented as the mean ± standard deviation. B) 3D scatter plots showing sperm DNA fragmentation (DFI) assessed by the TUNEL assay before and after exclusion of unstained cells by PI. If there was no difference, all points should line up on the Y axis. As the scatter for all 4 parameters monitored is evident, this suggests that the presence of M540 bodies is highly likely to influence the parameter measured

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Basic and Clinical Andrology

ISSN: 2051-4190