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Fig. 1 | Basic and Clinical Andrology

Fig. 1

From: Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation

Fig. 1

A) FSC/SSC dot plot identifying the flame shaped region (R1) containing spermatozoa and M540 bodies. B) Representative microscopic photograph showing a semen sample containing spermatozoa and M540 bodies (magnification: × 200). C) representative TUNEL and 8-OHdG flow cytometric charts of a sperm sample from the cohort having “normal sperm parameters” (left panels) and a sperm sample from the cohort having “abnormal sperm parameters” (right panels). Q4: TUNEL-8-OHdG positive/PI negative, Q3: PI negative/TUNEL-8-OHdG negative, Q2: PI-positive/TUNEL-8-OHdG positive, Q1: PI-positive/TUNEL-8-OHdG negative. Note: apoptotic or M540 bodies (arrows) appear in Q3

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Basic and Clinical Andrology

ISSN: 2051-4190