Table 2 Effect of supplementation of semen extender with different concentration of vitamin D on frozen–thawed spermatozoa quality (viability and motility) in normozoopsermic and asthenozoopsermic Holstein bulls

Total Motility*(%)

71.26 ± 2.83

65.58 ± 2.06

74.95 ± 9.75

82.68 ± 4.75

41.83 ± 12.37

60.60 ± 6.14

52.50 ± 2.45

48.30 ± 3.29

Progressive motility*(%)

53.90 ± 1.14^a

52.40 ± 1.44^a

55.63 ± 5.38^a

65.39 ± 1.56^b

20.52 ± 2.62 ^A

31.53 ± 0.86^B

32.29 ± 1.33^B

31.08 ± 0.14^B

Immotile*(%)

28.74 ± 2.83

34.42 ± 2.06

25.05 ± 9.75

22.03 ± 0.64

58.17 ± 12.37

39.40 ± 6.14

47.50 ± 2.45

51.70 ± 3.29

Viability (%)

54.33 ± 1.76^a

52.00 ± 0.58^a

56.33 ± 3.28^ab

63.00 ± 2.00^b

57.00 ± 3.06^B

45.67 ± 2.96^AB

37.67 ± 4.26 ^A

41.00 ± 5.00 ^A