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Table 2 Effect of supplementation of semen extender with different concentration of vitamin D on frozen–thawed spermatozoa quality (viability and motility) in normozoopsermic and asthenozoopsermic Holstein bulls

From: Effects of vitamin D supplementation in extender on sperm kinematics and apoptosis following the freeze-thaw process in normozoospermic and asthenozoospermic Holstein bulls

Vit D (ng/mL) Normozoospermic bull Asthenozoospermic bull
0 5 10 50 0 5 10 50
Total Motility*(%) 71.26 ± 2.83 65.58 ± 2.06 74.95 ± 9.75 82.68 ± 4.75 41.83 ± 12.37 60.60 ± 6.14 52.50 ± 2.45 48.30 ± 3.29
Progressive motility*(%) 53.90 ± 1.14a 52.40 ± 1.44a 55.63 ± 5.38a 65.39 ± 1.56b 20.52 ± 2.62 A 31.53 ± 0.86B 32.29 ± 1.33B 31.08 ± 0.14B
Immotile*(%) 28.74 ± 2.83 34.42 ± 2.06 25.05 ± 9.75 22.03 ± 0.64 58.17 ± 12.37 39.40 ± 6.14 47.50 ± 2.45 51.70 ± 3.29
Viability (%) 54.33 ± 1.76a 52.00 ± 0.58a 56.33 ± 3.28ab 63.00 ± 2.00b 57.00 ± 3.06B 45.67 ± 2.96AB 37.67 ± 4.26 A 41.00 ± 5.00 A
  1. Data are expressed as means ± SEM. a,b A, B Means in a row, with different capital alphabetic letters are significantly different P < 0.05
  2. * The effect of different concentration of vitamin D in normozoospermic bulls is significantly higher than asthenozoospermic bulls