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Table 1 Effect of supplementation of semen extender with different concentration of vitamin D on frozen–thawed spermatozoa kinematics in normozoopsermic and asthenozoopsermic Holstein bulls

From: Effects of vitamin D supplementation in extender on sperm kinematics and apoptosis following the freeze-thaw process in normozoospermic and asthenozoospermic Holstein bulls

Vit D (ng/mL)

Normozoospermic bull

Asthenozoospermic bull

0

5

10

50

0

5

10

50

DCL(µm)*

32.21 ± 1.82

32.21 ± 0.42

30.88 ± 2.04

33.46 ± 1.37

19.87 ± 3.38

21.92 ± 0.81

22.45 ± 2.44

21.77 ± 0.49

DSL (µm)*

12.96 ± 0.89

12.81 ± 0.45

11.93 ± 1.67

13.43 ± 1.07

6.76 ± 1.65

9.11 ± 0.36

9.12 ± 0.89

8.85 ± 0.71

DAP (µm)*

16.18 ± 0.98

16.17 ± 0.19

15.26 ± 1.55

16.87 ± 0.93

9.69 ± 1.94

11.89 ± 0.35

11.81 ± 1.13

11.69 ± 0.54

VCL(µm/s)*

88.45 ± 2.64ab

76.06 ± 1.11a

86.53 ± 6.95ab

97.15 ± 3.01b

47.05 ± 2.60

53.88 ± 1.99

55.98 ± 4.15

54.32 ± 2.60

VSL (µm/s) *

39.83 ± 1.10ab

32.51 ± 1.67a

37.48 ± 4.43ab

42.07 ± 1.92b

19.54 ± 1.42

24.11 ± 2.52

25.49 ± 1.37

24.63 ± 1.98

VAP (µm/s) *

47.43 ± 1.22ab

39.74 ± 1.43a

45.44 ± 4.45ab

50.94 ± 1.84b

25.77 ± 1.66

30.53 ± 2.38

31.47 ± 1.65

30.86 ± 1.79

BCF(Hz)*

11.77 ± 0.70

10.79 ± 0.13

12.04 ± 1.05

12.71 ± 0.45

6.77 ± 1.02

8.71 ± 0.38

7.86 ± 1.02

7.93 ± 0.61

ALH(µm)*

1.10 ± 0.06

1.02 ± 0.02

1.04 ± 0.07

1.16 ± 0.04

0.64 ± 0.05

0.71 ± 0.02

0.73 ± 0.06

0.69 ± 0.03

STR(%)

0.84 ± 0.00

0.82 ± 0.01

0.82 ± 0.02

0.83 ± 0.01

0.76 ± 0.01

0.78 ± 0.02

0.81 ± 0.01

0.79 ± 0.02

LIN(%)

0.45 ± 0.00

0.43 ± 0.02

0.43 ± 0.02

0.43 ± 0.01

0.40 ± 0.01

0.45 ± 0.03

0.46 ± 0.01

0.46 ± 0.03

WOB(%)

0.53 ± 0.00

0.52 ± 0.01

0.53 ± 0.01

0.52 ± 0.00

0.52 ± 0.00

0.57 ± 0.02

0.56 ± 0.01

0.57 ± 0.02

  1. Values are presented as mean ± SEM. ALH anterior lateral head displacement; BCF beat cross frequency; DSL straight-line distance; DAP average path distance; DCL curvilinear distance; LIN linearity; STR straightness; VSL straight-line velocity; VAP average path velocity; VCL curvilinear velocity; WOB wobble.* The effect of different doses of vitamin D in normozoospermic bulls is significantly greater than that in asthenozoospermic bulls.a, b Means in a row, with different capital alphabetic letters are significantly different P < 0.05. Means without common subscript(s) are not significantly different (P > 0.05)