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Fig. 1 | Basic and Clinical Andrology

Fig. 1

From: Effects of vitamin D supplementation in extender on sperm kinematics and apoptosis following the freeze-thaw process in normozoospermic and asthenozoospermic Holstein bulls

Fig. 1

Evaluation Intact acrosome integrity (PMI) of frozen-thawed normozoospermic and asthenozoospermic Holstein bulls spermatozoa supplemented with different concentration of vitamin D. Evaluation Plasma membrane integrity (AI) of frozen-thawed normozoospermic and asthenozoospermic Holstein bulls spermatozoa supplemented with different concentration of vitamin D. *Data are expressed as means ± SEM. a, b, A, B Different alphabetic letters in each bar indicate statistically significant differences in normozoospermic and asthenozoospermic bulls, respectively. ** There is no significant difference between the effects of different concentrations of vitamin D in asthenozoospermic and normozoospermic bulls, although there is a significant difference between the effects of different concentrations in each group of bulls.

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