Fig. 4
From: LRWD1 expression is regulated through DNA methylation in human testicular embryonal carcinoma cells
The regulation of LRWD1 expression by methylation activators and inhibitors in NT2/D1 cells. To detect the effect of (a) a methylation activator, floxuridine and (b) a methylation inhibitor 5-Aza-dc on LRWD1 expression, NT2/D1 cells were treated with 1, 3, 5 and 10 μM floxuridine or 5-Aza-dc for 24 h. Total lysates were evaluated by Western blotting. The expression levels of LRWD1 were quantified using ImageJ software. The data represent the mean of LRWD1 protein expression level from three independent experiments. Columns, mean; bars, SD (n = 3). Significant differences (*, P < 0.05; **, P < 0.01 and ***, P < 0.001, one-way ANOVA) between the control and experimental group are marked with asterisks. 5-Aza-dc, 5-Aza-2′-deoxycytidine. LRWD1, Leucine-rich repeat and WD repeat-containing protein 1. SD, Standard deviation