Fig. 2
From: LRWD1 expression is regulated through DNA methylation in human testicular embryonal carcinoma cells
The reduction of LRWD1 promoter activity by DNA methylation in NT2/D1 cells. Two pCpGL-basic and pCpGL-hLRWD1 plasmids were treated with or without methyltransferase. After DNA methylation assay, the methylated or unmethylated plasmids were transfected into (a) NT2/D1 and (b) Hela cells. The promoter activity was assessed by luciferase reporter gene assay, and luciferase activities were normalized to pRL-TK reporter activities and presented as fold induction compared with the empty vector (control). Columns, mean of three independent experiments; bars, SD (***, p < 0.001, one-way ANOVA). pRL-TK, the thymidine kinase promoter-Renilla luciferase reporter plasmid. LRWD1, Leucine-rich repeat and WD repeat-containing protein 1. SD, Standard deviation