Fig. 6
From: Is ferroptosis involved in ROS-induced testicular lesions in a varicocele rat model?
Relative protein expression between groups. Legend: a Relative protein expression of Nrf2, Slc7a11, Gpx-4 and p-Jnk between groups at 2 months. b Relative protein expression of Nrf2, Slc7a11, Gpx-4 and p-Jnk between groups at 4 months. c Western blot bands representative for Nrf2, Slc7a11, Gpx-4, p-Jnk and ß-actin as housekeeping protein in 2 and 4 months groups. A minimum of 3 replicates were carried out. Evaluation of the relative expression of Nrf2 showed a significant increase in the V.I-ALA+ group compared to the V.I-ALA− group. Evaluation of the relative expression of p-Jnk showed a significant increase in the 2 M varicocele group compared to the 2 M control group. ALA: Alpha lipoic acid, Gpx-4: glutathione peroxidase-4, M: month post-surgery or varicocele induction (V.I), Nrf2: nuclear factor erythroid 2–related factor, p-Jnk: phosphorylated c-Jun N-terminal kinase, V.I-ALA+: varicocele rats treated with ALA+ for 2 to 4 months post induction of varicocele, V.I-ALA−: varicocele rats treated with water as solvent of ALA for 2 to 4 months post induction of varicocele. All data are presented as Mean ± SEM. p-value is provided for the groups which showed significant difference