Skip to main content
Fig. 4 | Basic and Clinical Andrology

Fig. 4

From: Excessive unilateral proliferation of spermatogonia in a patient with non-obstructive azoospermia – adverse effect of clomiphene citrate pre-treatment?

Fig. 4

Immunohistochemical analysis of cell proliferation (a, b) and cell death (c, d). Pictures a and c show intact spermatogenesis (NSP) as control, pictures b and d show spermatogonial arrest (patient’s left testis). a, b Proliferation was tested by PCNA immunostaining and revealed intensely stained spermatogonia (black arrows) and primary spermatocytes (black arrowheads). In the spermatogonial arrest tubules, PCNA staining was detected in more spermatogonia compared to NSP. c, d TUNEL staining revealed apoptotic germ cells in NSP (red arrows) and to a higher extend in tubules with abundant spermatogonia. AEC detection, bar 50 μm (inset bar 25 μm), inset as negative control without first antibody

Back to article page

Basic and Clinical Andrology

ISSN: 2051-4190