TY - JOUR AU - Mohammadi, Z. AU - Tavalaee, M. AU - Gharagozloo, P. AU - Drevet, J. R. AU - Nasr-Esfahani, M. H. PY - 2020 DA - 2020/08/13 TI - Could high DNA stainability (HDS) be a valuable indicator of sperm nuclear integrity? JO - Basic and Clinical Andrology SP - 12 VL - 30 IS - 1 AB - The Sperm Chromatin Structure Assay (SCSA®), in addition to identifying the DNA Fragmentation Index (DFI) also identifies High DNA satiability (HDS), supposed to reflect the nuclear compaction of spermatozoa. However, data on what exactly this parameter reveals, its relevance and usefulness are contradictory. In order to shed light on this situation, spermatozoa of a cohort (N = 397) of infertile men were subjected to the SCSA®, TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling) and CMA3 (Chromomycin A3) tests. In a smaller subcohort (N = 100), aniline blue (AB) and toluidine blue (TB) staining were performed in addition. The objective of this study was thus to answer the question of whether HDS is a relevant and reliable parameter to be taken into account? SN - 2051-4190 UR - https://doi.org/10.1186/s12610-020-00110-8 DO - 10.1186/s12610-020-00110-8 ID - Mohammadi2020 ER -