Can the SCD test and terminal uridine nick-end labeling by flow cytometry technique (TUNEL/FCM) be used interchangeably to measure sperm DNA damage in routine laboratory practice?

Table 2 Assessment of reliability of DNA damage (in percentages)

Reliability analysis	Effect	N	Mean difference (SE)	p-value*	ICC^a
Within SCD	Reading	200	− 0.205 (0.70)	0.3975	0.96632
	Reader	100	0.816 (1.25)	0.8213	0.94680
	Slide	50	−1.142 (1.08)	0.5195	0.98251
Between SCD and TUNEL	Technique	50	−3.392 (1.45)	0.0127	0.93834

Reliability analysis within SCD test and inter-method reliability between the SCD test and the TUNEL assay
*p-value of the signed-rank test
^aICC Intraclass Correlation Coefficient