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Table 1 Mean values of studied parameters and comparisons between groups

From: Protective role of N-acetylcysteine (NAC) on human sperm exposed to etoposide

Parameters TPM (%) HOST (%) AB+ (%) TUNEL (%) 8-OHdG (%)
Mean values
 CT 57.0 ± 6.0 50.7 ± 5.3 21.8 ± 9.5 16.1 ± 3.2 9.3 + 4.4
 NAC 68.6 ± 9.2 49.9 ± 5.2 22.2 ± 11.4 15.4 ± 2.4 23.7 ± 13.5
 ETO 60.1 ± 7.1 56.4 ± 6.6 33.7 ± 11.1 23.7 ± 1.1 24 ± 16.5
 NAC-ETO 49.3 ± 9.9 47.5 ± 4.8 23.2 ± 6.6 20.4 ± 4.5 26 ± 12
 ETO-NAC 50.2 ± 5.2 49.4 ± 8.0 20.8 ± 9.0 19.1 ± 6.3 25.6 ± 11.7
Statistical comparisons between groups (P values)
 CT vs NAC NS NS NS NS 0.002
 CT vs ETO NS NS 0.02 0.0019 0.002
 CT vs NE NS NS NS 0.0044 0.0421
 CT vs EN NS NS NS NS 0.0283
 NAC vs ETO NS 0.04 0.04 0.02 NS
 NAC vs NE NS NS NS 0.0039 NS
 NAC vs EN NS NS NS 0.0042 NS
 ETO vs NE NS 0.01 0.0001 NS NS
 ETO vs EN NS 0.04 0.00001 NS NS
 NE vs EN NS NS 0.0044 NS NS
  1. Values are expressed in mean ± standard deviation, TPM = sperm total progressive motility, HOST = sperm hypoosmotic swelling test, AB+ = positive sperm aniline blue staining (presence of immature chromatin), TUNEL = terminal deoxynucleotidyl transferase dUTP nick end labelling (presence of sperm DNA fragmentation), 8-OHdG = 8-hydroxy-2′-deoxyguanosine (presence of sperm oxidative damage), CT = control, NAC = N-acetylcysteine, ETO = etoposide, NAC-ETO = incubation with NAC followed by etoposide addition, ETO-NAC = incubation with etoposide followed by addition of NAC, Significant differences (P < 0.05), NS = not significant