Protective role of N-acetylcysteine (NAC) on human sperm exposed to etoposide

Table 1 Mean values of studied parameters and comparisons between groups

Parameters	TPM (%)	HOST (%)	AB+ (%)	TUNEL (%)	8-OHdG (%)
Mean values
CT	57.0 ± 6.0	50.7 ± 5.3	21.8 ± 9.5	16.1 ± 3.2	9.3 + 4.4
NAC	68.6 ± 9.2	49.9 ± 5.2	22.2 ± 11.4	15.4 ± 2.4	23.7 ± 13.5
ETO	60.1 ± 7.1	56.4 ± 6.6	33.7 ± 11.1	23.7 ± 1.1	24 ± 16.5
NAC-ETO	49.3 ± 9.9	47.5 ± 4.8	23.2 ± 6.6	20.4 ± 4.5	26 ± 12
ETO-NAC	50.2 ± 5.2	49.4 ± 8.0	20.8 ± 9.0	19.1 ± 6.3	25.6 ± 11.7
Statistical comparisons between groups (P values)
CT vs NAC	NS	NS	NS	NS	0.002
CT vs ETO	NS	NS	0.02	0.0019	0.002
CT vs NE	NS	NS	NS	0.0044	0.0421
CT vs EN	NS	NS	NS	NS	0.0283
NAC vs ETO	NS	0.04	0.04	0.02	NS
NAC vs NE	NS	NS	NS	0.0039	NS
NAC vs EN	NS	NS	NS	0.0042	NS
ETO vs NE	NS	0.01	0.0001	NS	NS
ETO vs EN	NS	0.04	0.00001	NS	NS
NE vs EN	NS	NS	0.0044	NS	NS

Values are expressed in mean ± standard deviation, TPM = sperm total progressive motility, HOST = sperm hypoosmotic swelling test, AB+ = positive sperm aniline blue staining (presence of immature chromatin), TUNEL = terminal deoxynucleotidyl transferase dUTP nick end labelling (presence of sperm DNA fragmentation), 8-OHdG = 8-hydroxy-2′-deoxyguanosine (presence of sperm oxidative damage), CT = control, NAC = N-acetylcysteine, ETO = etoposide, NAC-ETO = incubation with NAC followed by etoposide addition, ETO-NAC = incubation with etoposide followed by addition of NAC, Significant differences (P < 0.05), NS = not significant

ISSN: 2051-4190