Table 2 Changes in expression of genes involved in innate and adaptive immune responses after 3 and 6 h of LPS-induced inflammation
Gene Name | Fold Change | Gene Functions | |
|---|---|---|---|
3 h | 6 h | ||
Complement component 3 (C3) Rn.11378, NM_016994 | No change | 7.72 (p = 0.002) | Activation of the complement system. |
Chemokine (C-C motif) ligand 12 (Cc112); Rn.137780, NM_001105822 | 8.52 (p = 0.000) | 26.15 (p = 0.035) | Angiogenesis; cellular response to fibroblast growth factor stimulus; participates in chemokine and cytokine mediated and cytokine signaling pathways. |
Chemokine (C-C motif) ligand 3 (Cc13) Rn.10139, NM_013025 | 10.62 (p = 0.000) | 14.63 (p = 0.043) | Monokine with inflammatory and chemokinetic properties. |
CD 14 molecule (Cd14) Rn.42942, NM_021744 | 7.17 (p = 0.002) | 6.67 (p = 0.002) | Accessory protein for TLR4. |
Chemokine (C-X-C motif) ligand 10 (Cxc110); Rn.10584, NM_139089 | 24.23 (p = 0.000) | NSS | Proinflammatory cytokine; may participate in T-cell effector function and development. Inhibits expression of StAR D1. |
Intercellular adhesion molecule 1 (1cam1) Rn. 12, NM_012967 | 25.26 (p = 0.000) | NSS | Up-regulated by TLR2, TLR4, TLR5, and TLR6; ligand for leukocyte adhesion protein. |
Interleukin 10 (1110) Rn.9868, NM_012854 | 4.43 (p = 0.021) | No change | Inhibits cytokine synthesis. |
Interleukin 1 beta (111b) Rn.9869, NM_031512 | 11.32 (p = 0.000) | 14.18 (p = 0.053) | Endogenous pyrogen, proinflammatory cytokine. |
Interleukin 6 (116) Rn.9873, NM_012589 | 69.61 (p = 0.000) | NSS | Disrupts integrity of blood-testis-barrier by altering steady state levels of membrane proteins. |
Janus kinase 2 (Jak2) Rn.18909, NM_031514 | No change | 3.77 (p = 0.032) | Non-receptor tyrosine kinase known to be involved in innate and adaptive immunity signaling. |
Nuclear factor of kappa light polypeptide gene (Nfkbia) Rn.12550, NM_001105720 | 4.84 (p = 0.000) | 3.99 (p = 0.018) | Inhibitor of NF-ĸB; binds NF-ĸB and retains it in the cytoplasm. |
NLF family, pyrin domain containing 3 (Nlrp3) Rn.214177, XM_220513 | No change | 8.27 (p = 0.001) | No functional assignments in Uniprot. |
Solute carrier family 11 (proton-coupled divalent; Slc11a1) Rn.105919, NM_001031658 | No change | 3.79 (p = 0.000) | Macrophage protein associated with resistance or susceptibility to intracellular pathogens. |
Toll-like receptor 1 (Tlr1) Rn. 107,212, NM_001172120 | No change | 3.36 (p = 0.018) | Stimulated by bacterial lipoproteins. |
Toll-like receptor 2 (Tlr2) Rn. 46,387, NM_198769 | 6.35 (p = 0.000) | 8.70 (p = 0.027) | Increase expression of inflammatory cytokines IL-α, IL-6, and interferon-α, and β |
Toll-like receptor 5 (Tlr5) Rn. 198,962, NM_001145828 | −3.37 (p = 0.052) | No change | Increase expression of inflammatory cytokines IL-α, IL-6, and interferon-α, and β |
Tumor necrosis factor (TNF superfamily, member 2; Tnf) Rn.2275,NM_012675 | 8.78 (p = 0.000) | 4.58 (p = 0.029) | Chemokine-mediated signaling, induces germ cell apoptosis in autoimmune orchitis. |