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Table 2 Studies of the seminal plasma proteomic profile in different biological conditions

From: Understanding the seminal plasma proteome and its role in male fertility

Author Biological condition Study design Identified and differentially expressed proteins Main expressed proteins Main molecular functions
Zylbersztejn et al. 2013 [56] Varicocele - 67 adolescents in total: - 21 without varicocele and normal SA; - 28 with varicocele and normal SA; - 18 with varicocele and abnormal SA. From 181 to 323 spots per gel: - 31 proteins in adolescent without varicocele; - 7 proteins in adolescent with varicocele and normal SA; - 11 proteins in adolescents with varicocele and abnormal SA - Adolescent without varicocele: SEMG1, PIP and NPC2 - Adolescent with varicocele and normal SA: EP3B, SEMG2, SAP and BRE1B - Adolescent with varicocele and abnormal SA: CYTS, PPAP and IBP3. - Spermatogenesis - Transport of molecules - Immune response - Inflammatory pathways - Apoptosis
Del Giudice et al. 2013 [59] Varicocele 19 adolescents before and after varicocelectomy From 84 to 168 spots per gel: - 4 proteins overexpressed in pre-varicocelectomy adolescents - - Overexpressed in Pre: Albumin, POLR3B, PIP and NELFE - Exclusive in Post: SEMG2, SEMG1, PIP, PSA, TGM4, SERPINA1 - Sperm-binding - Metal-iron-binding - Innate immune response
Camargo et al. 2013 [55] Varicocele 18 adult men before and after varicocelectomy - 316 proteins identified in total: - 58 proteins overexpressed or exclusive in pre-varicocelectomy - 38 proteins exclusive in post-varicocelectomy - Pre-varicocelectomy: ANXA5, LDHA, CLU, HSP90AB1 and HSP90AA1 - Post-varicocelectomy: DJ1, SOD1, S100A9, ANXA1, GAPDH and MDH1. - Cellular response to ROS - NAD-binding - Nitric oxide metabolic process - TPR domain - Eicosanoid biosynthetic process
Del Giudice et al. 2016 [57] Varicocele - 77 adolescents in total: - 23 adolescents without varicocele; - 37 adolescents with varicocele and normal SA; - 17 adolescents with varicocele and abnormal SA. - 541 proteins in total: - 108 overexpressed in adolescents without varicocele; - 26 overexpressed in adolescent with varicocele and normal SA; - 13 overexpressed in varicocele and abnormal SA. - Candidate biomarkers: - Adolescents without varicocele: SDF4, LEFTY1, DNASE1 and PLPP1; - Adolescents with varicocele and normal SA: IGFBP7 and IGHG3; - Adolescents with varicocele and abnormal SA: CRISP3 - hexose metabolic process - secretory granule - wound healing - lysosome
Fariello et al. 2012 [60] Smokers with varicocele - 56 adult nonsmokers with varicocele; - 29 adult moderate smokers with varicocele; - 25 adult heavy smokers with varicocele. - 20 identified proteins: - 4 proteins in nonsmokers - 5 proteins in moderate smokers - 1 protein in heavy smokers - Nonsmokers: SERPINA1, ASAH1, CALM1, CRISP1; - Moderate smokers: ANXA3, CTSB, EDDM3B, PTGDS, SOD3; - Heavy smokers: AZGP1 - Lipid catabolic process - Inflammatory pathways - Proteolysis - Apoptosis
Antoniassi et al. 2016 [61] Smokers - 20 nonsmoking men; - 20 smoking men. - 422 identified and quantified proteins: - 1 absent; - 6 over-represented; - 27 under-represented. IGLC3, IGHA1, APOA1, S100A9, ORM1, LCN2 and SCGB2A1 - antigen processing and presentation - protein kinase A signaling and arachidonic acid secretion - complement activation - positive regulation of antigen processing and presentation - regulation of acute inflammatory response
Da Silva et al. 2013 [54] Spinal Cord Injured - 12 SCI men (6 collected by EEJ and 6 collected by PVS); - 10 healthy control donor. - 637 identified proteins: - 35 exclusive in control group - 88 exclusive in PVS group - 66 exclusive in EEJ group PVS proteins of interest: TRFL, CERU, C3, CPB, NFAC4, EP300, IGKC EEJ proteins of interest: SPTA2 and SYNE1 - cytoskeletal binding - response to calcium - oxidation of iron ion
Da Silva et al. 2016 [53] Spinal Cord Injured - 12 SCI men; - 11 healthy control donor. - 2550 proteins identified in total: - 344 exclusive in SCI group; - 146 proteins exclusive in controls PRTN3, ELANE, DEFA1, PZP and A2M - inflammatory processes; - protease inhibition;
Camargo et al. 2015 [62] Spinal Cord Injured - 9 SCI men before and after oral treatment with probenecid - 806 proteins in total: - 77 proteins over-expressed or exclusive pre-treatement - 33 exclusive or over-expressed after the treatment. Pre-treatment: NEDD8, CUL3, PSMD7, PSMA8 and PMSB7. Post-treatment: GARS and GART. - proteasomal degradation - ubiquitination - inflammatory activity
Milardi D. et al. 2014 [52] Male Hypogonadism - 10 men with secondary hypogonadism - 10 fertile and normogonadic men - 61 proteins identified in fertile group - of which, 33 were absent in hypogonadic patients - 14 proteins found in hypogonadism men after therapy; Therapy related proteins: S100A9, PIP, AZGP1, ACPP, LTF, MPO, CST3 - catalytic activity - binding activity - hydrolase activity
  1. - SA semen analysis
  2. - SCI spinal cord injured