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Fig. 1 | Basic and Clinical Andrology

Fig. 1

From: Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men

Fig. 1

Immunostaining of representative samples of human ejaculated spermatozoa with Hs-14 monoclonal antibody and corresponding phase-contrast microphotographs. The antibody strongly labelled sperm acrosome (a, b). In some samples, apart from the acrosome, the flagellum of spermatozoa was also stained (b). Pictures of a series of 3-μm distributed sections of sperm cell in confocal microscope. The 3rd to the 5th sections show the highest staining (c). Blue colour – DAPI staining of the nucleus, green colour – FITC staining (Fig. 1a and 1b magnification 600×, Fig. 1c magnification 1000×)

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