Outcomes with intracytoplasmic sperm injection of cryopreserved sperm from men with spinal cord injury

Table 2 Parameters of semen obtained by antegrade ejaculation before freezing and after thawing (n=10)

Patient no	1	4	7	10	11	12	13	16	17	18	Mean	SD
Parameters before freezing
No of retrieval (s)	1	1	3	1	1	2	1	2	2	1	1.5	0.7
Volume (mL)	5.4	0.15	0.6	0.1	1.3	0.45	3.4	3.4	0.3	2	2	1.7
Concentration (×10 ⁶ /mL)	112	280	19	5	40	0.055	78	35.8	38	210	82	88.9
Total sperm count (10 ⁶⁾	605	42	11	1	52	0.025	265	122	11	420	153	199.6
PM (a+b) %	42	20	19	0	23	drop	13	35	33	34	24	12.3
TM (a+b+c) %	49	29	37	3	28	drop	19	48	54	40	34	15.3
Viability %	52	38	50	38	62	nd	42	62	66	53	51	9.9
Morphology %	51	10	36	4	7	nd	31	35	27	23	25	14.6
number of sperm straws	19	5	11	2	5	5	12	29	10	10	11	7.6
No of spz per straw (M)	20	2.6	4.3	0.6	3.6	0.01	12	7.2	5.8	18.3	16.8	4.2
Parameters after thawing
PM (a+b) %	10	8	3.3	drop	13	drop	1	22.5	0	14	7.7	6.0
TM (a+b+c) %	20	11	8	drop	21	drop	2	31	16.5	25	16.9	10.2
Viability %	52	38	24	38	62	nd	17	47	30	53	40.1	14.7

Antegrade ejaculation group composed by 9 patients with PVS and one patient after sexual intercourse (patient 11).
nd (not determined) which means not enough spermatozoa to perform the analysis; drop: the number of spermatozoa was determined on several 10 μl drops of sperm suspension covered with mineral oil.
PM: progressive motility; TM: total motility; morphology: French classification David % [25]; SD: standard deviation, No: number; spz: spermatozoa; M: million.

ISSN: 2051-4190