Expression and localization of aromatase during fetal mouse testis development

Table 1 Sequences of aromatase primers used in RT-PCR and qRT-PCR

		Sequence 5′-3′	Tm
RT-PCR all transcripts	forward	AACCCCATGCAGTATAATGTC	55°C
RT-PCR all transcripts	reverse	CATTCTTCTCAAAGTTTTCA	55°C
T1 qRT-PCR	forward	GCCTCCTTCTCCTGATTTGGA	60°C
	reverse	CTGCCATGGGAAATGAGGG
	internal probe	TACCAGGTCCTGGCTACT
T2 qRT-PCR	forward	GCCTCCTTCTCCTGAATTTGGA	60°C
	reverse	CCGAATCGGGAGATGTAGTGA
	internal probe	TCAATACCAGGTCCTCAAGC
T3 qRT-PCR	forward	CCATGCCACTCCTGCTGAT	60°C
	reverse	CCACCATTCGAACAAGACCAG
	internal probe	TCTTCAATACCAGCTCTGACGGGCC

ISSN: 2051-4190