Fig. 7
From: External quality assessment scheme for sperm DNA fragmentation: a pilot study in China
A Dot plot with fixed gating analysis for flow cytometry. The fixed gating of the flow cytometry analysis software was biased to the right, and the result of sperm DNA fragmentation was reduced. B Dot plot with manual gating analysis for flow cytometry. Sperm with abnormal DNA fragmentation can be well included by manual gating. PerCP-Cy5.5: red AO fluorescence of sperm with broken DNA; FIFC: green AO fluorescence of DNA stainability