Fig. 6
From: External quality assessment scheme for sperm DNA fragmentation: a pilot study in China
A Dot plot with a sample size of 25 µl for flow cytometry. When 25 µl of the semen sample was added, the denaturation effect of the low-pH (1.2) detergent solution on sperm chromatin was weakened, and the result of sperm DNA fragmentation was low. B Dot plot with a sample size of 5 μl for flow cytometry. When 5 μl of the semen sample was added, the acid denaturation effect of the acid treatment solution was good and the result of sperm DNA fragmentation was accurate. PerCP-Cy5.5: red AO fluorescence of sperm with broken DNA; FIFC: green AO fluorescence of DNA stainability