Patient’s Age classes
N =
|
20–29
721
|
30–39
5736
|
40–49
2990
|
50–59
241
|
P
-Value
|
---|
SDF-TUNEL (%) | 8.39 ± 4.03a | 9.47 ± 5.34b | 11.1 ± 6.82c | 13.9 ± 8.63d | < 2.2 e-16 |
SDF-SCSA (%) | 16 ± 5.83a | 17.7 ± 7.16b | 19.9 ± 8.52c | 23.8 ± 10.14d | < 2.2 e-16 |
HDS SCSA (%) | 8.22 ± 4.31 | 8.54 ± 4.23 | 8.47 ± 4.06 | 8.02 ± 3.61 | 0.12 |
Ratio SDF-SCSA/SDF-TUNEL | 1.91 | 1.87 | 1.79 | 1.71 | - |
- The patients were divided into four groups according to age. Patients over age 59 years were not included in the analysis since this group contains only 46 individuals. Mean ± standard deviation of all parameters is indicated
- For each variable, Kruskall-Wallis test was used to determine whether or not there is a statistically significant difference between the medians of the different groups of male age patients. The significance level was set to P < 0.05. When significant, a Dunn’s test was conducted (Holm adjustment) to determine which groups are different. Different lower-case letters indicate a significant difference among Patient’s groups
-
HDS High DNA Stainability, SCSA Sperm Chromatin Structure Assay, SDF Sperm DNA Fragmentation, TUNEL Terminal deoxynucleotidyl transferase dUTP Nick End Labeling