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Table 2 Comparison of sperm DNA fragmentation indexes between age classes of male patients

From: Sperm chromatin structure assay (SCSA®) and flow cytometry-assisted TUNEL assay provide a concordant assessment of sperm DNA fragmentation as a function of age in a large cohort of approximately 10,000 patients

Patient’s Age classes

N = 

20–29

721

30–39

5736

40–49

2990

50–59

241

P -Value

SDF-TUNEL (%)

8.39 ± 4.03a

9.47 ± 5.34b

11.1 ± 6.82c

13.9 ± 8.63d

 < 2.2 e-16

SDF-SCSA (%)

16 ± 5.83a

17.7 ± 7.16b

19.9 ± 8.52c

23.8 ± 10.14d

 < 2.2 e-16

HDS SCSA (%)

8.22 ± 4.31

8.54 ± 4.23

8.47 ± 4.06

8.02 ± 3.61

0.12

Ratio

SDF-SCSA/SDF-TUNEL

1.91

1.87

1.79

1.71

-

  1. The patients were divided into four groups according to age. Patients over age 59 years were not included in the analysis since this group contains only 46 individuals. Mean ± standard deviation of all parameters is indicated
  2. For each variable, Kruskall-Wallis test was used to determine whether or not there is a statistically significant difference between the medians of the different groups of male age patients. The significance level was set to P < 0.05. When significant, a Dunn’s test was conducted (Holm adjustment) to determine which groups are different. Different lower-case letters indicate a significant difference among Patient’s groups
  3. HDS High DNA Stainability, SCSA Sperm Chromatin Structure Assay, SDF Sperm DNA Fragmentation, TUNEL Terminal deoxynucleotidyl transferase dUTP Nick End Labeling