Fig. 4
From: Dnali1 is required for sperm motility and male fertility in mice
Intracytoplasmic sperm injection overcomes the infertility caused by Dnali1 deficiency. (A-B) Representative 4.5-day blastocysts (A) and the blastocyst rate (B) to evaluate the ability of sperm in reaching oocytes for fertilization in vivo. Female WT mice were superovulated and mated with adult Dnali1+/+ and Dnali1−/− male mice; embryos were collected from female mice at the zygote stage and then were cultured to the blastocyst stage. Scale bar, 100 μm. ***P < 0.001, unpaired Student’s t-test. C Representative offspring obtained from ICSI using sperm from Dnali1+/+ and Dnali1−/− male mice. D Sequencing chromatograms of ICSI offspring from Dnali1+/+ and Dnali1−/− male mice. E The rates of ICSI embryos developing into two-cell embryos and live-born pups. ICSI embryos at the two-cell stage were transferred into the oviducts of pseudopregnant female mice. WT, Wild-type; ICSI, Intracytoplasmic sperm injection