Fig. 1
From: Dnali1 is required for sperm motility and male fertility in mice
Dnali1 is a conserved and testis-enriched gene whose deficiency causes male infertility. A Gene expression levels of Dnali1 in the indicated WT mouse tissues. DNA marker was shown in the first lane. B Gene expression analysis showing Dnali1 mRNA levels in the developing postnatal WT mouse testes. Gapdh was used as the internal control. DNA marker was shown in the first lane. C An interspecies protein alignment from the Uniport database, showing high conservation of DNALI1 in mammals. D Sequencing chromatograms of Dnali1+/+ and Dnali1−/− mouse gDNA showing the intended nonsense mutation in the Dnali1−/− mouse. E Immunoblotting showing the absence of DNALI1 protein (with no truncated protein) in the testis of Dnali1−/− male mice. Two types of antibodies against DNALI1 were used (Antibody 1: Proteintech-17601–1-AP; Antibody 2: Abcam-ab155490). n = 3 mice for both the Dnali1+/+ and Dnali1−/− groups respectively. α-TUBULIN was used as a loading control. F Fecundity testing of adult Dnali1+/+ and Dnali1−/− male mice during a 3-month observation window. The average litter size was calculated by dividing total numbers of pups by the total numbers of litters. ***P < 0.001, unpaired Student’s t-test. WT: Wild-type