Fig. 4
From: Cooperative cell–cell actin network remodeling to perform Gap junction endocytosis
Actin organization in C-shaped and U-shaped plaques analysed at the ultrastructure level associated with enhanced local contrast and skeletonization. A Careful examination after enhanced contrast of a selected region of a C-shaped plaque (upper left panel), indicated in the inset (upper right panel), demonstrates the presence of fine anastomosed filaments structuring a large network (arrows). The binary 8 bit (lower left panel) and the final skeletonized images (lower right panel) of the same region highlight the presence of fine actin meshes composing the network (lower left and right panels, arrows). B Similar image manipulation of a U-shaped plaque confirms that the actin network covers the entire structure of the plaque (arrows)