Fig. 2
From: Cooperative cell–cell actin network remodeling to perform Gap junction endocytosis
Electron microscopy analysis of actin organization during GJP endocytosis A Image in cross section of part of a GJP trilaminar structure reveals the peripheral irregular festoon of actin (left panel, arrows). ImageJ Enhanced Contrast clarifies the spiny structure of the actin decorating both sides of the GJP (right panel, arrows). B Semiquantitative analysis of actin-like spines decorating the GJP during the different steps of endocytosis before AGJ formation shows that the number of fine filaments present on Donor or Acceptor cells side is not significantly different. More than one hundred images in each cases. C Electron microscopy image of a U-Shaped plaque (left panel). Careful examination reveals that fine elongated actin filaments are oriented towards the cytoplasm and are mainly attached to the donor side of the GJP edge (right panel, inset 1, arrowheads), whereas numerous short actin filaments emanate from the acceptor side of the GJP (inset 2, arrows)