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Fig. 1 | Basic and Clinical Andrology

Fig. 1

From: Screening of sperm antigen epitopes by phage display technique and its preliminary clinical application

Fig. 1

Positive phage clones (blue plaque) on an LB/X-gal/IPTG plate. The Ph.D.-12 phage display peptide library was panned by the microtitre plate coated with AsAb-IgG, and the eluted phages were further adsorbed with AsAb negative-IgG. Then, the adsorbed phages were mixed with Escherichia coli ER2738 and cultured overnight. The culture mixture was quickly mixed with preheated top agar, and immediately added onto the LB plate containing X-gal and isopropyl-beta-D-thiogalactoside (IPTG) for screening the blue bacterial colonies with bacteriophages. The blue dots in the figure were positive phage clones

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Basic and Clinical Andrology

ISSN: 2051-4190