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Fig. 3 | Basic and Clinical Andrology

Fig. 3

From: Effects of lipopolysaccharide-induced inflammation on hypoxia and inflammatory gene expression pathways of the rat testis

Fig. 3

Phospho-NF-κB and IκB protein levels do not change following LPS-induced inflammation in rat testis. a Results of phospho-NF-κB and phospho-IκB immunoblot analysis of testicular cytoplasmic proteins isolated from sham and LPS treated animals. RAW cell line protein extract was included as a positive control and ß-actin was included as an internal control. Representative time points and results are shown in panel (a). Histograms show relative phospho-IκB (b) and phospho-NF-κB (c) protein levels normalized to ß-actin and as compared to sham for all time points and treatment samples analyzed. No statistically significant differences in phospho-IκB and phospho-NF-κB protein levels were observed between sham and LPS treated groups (ANOVA, P < 0.05; n = 3–5). STD, protein molecular weight standards

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Basic and Clinical Andrology

ISSN: 2051-4190