Fig. 2

HIF-1α protein levels increase following LPS-induced inflammation in rat testis. a Results of HIF-1α immunoblot analysis of testicular cytoplasmic proteins isolated from sham and LPS treated animals. COS-7 cell line protein extract was included as a positive control and ß-actin was included as an internal control. HIF-1α was detected at ~ 120 kDa. Shown is a 6 h sham as an example but time-matched shams (1, 3 and 6 h) were used for all experiments and for quantitation. Representative time points and results are shown in panel (a). b Histogram shows relative HIF-1α protein levels normalized to ß-actin and as compared to sham for all time points and treatment samples analyzed. *Indicates statistically significant difference as compared to sham (ANOVA, P < 0.05; n = 4–7). **Indicates P < 0.001